Figure 1.
Typical growth curve and the three growth parameters derived from the curve.
Figure 2.
For each plate, 32 samples (SA) and 32 standards were prepared. Stock A was used for preparing 32 samples on plate PA. * Milli-Q water was used instead of heavy water to prepare stock solution for the preparation of standards.
Table 1.
Stock solutions and their corresponding D content in the final samples.
Figure 3.
Maximum growth rate, lag time, maximum density of E. coli grown in minimal media.
(A) Blue circles: maximum growth rate of E. coli grown in M9 minimal media normalized by that at normal deuterium content of 156 ppm. * denotes p<0.05, ** - p<0.005, etc. Brown squares: predicted maximum growth rate calculated according to the maximum growth rate of E. coli grown in 50% of deuterium. (B) Blue circles: lag time of E. coli grown in M9 minimal media normalized by that at terrestrial content of deuterium from 156 ppm (terrestrial value) to 8%. Inset shows a zoom-in of the ultralow enrichment region. Brown squares: predicted lag time calculated according to the lag time of E. coli grown in 50% of deuterium. (C) Blue circles: maximum density of E. coli grown in M9 minimal media normalized by that at terrestrial deuterium content from 156 ppm (terrestrial value) to 8%. Inset shows a zoom-in of the ultralow enrichment region. Brown squares: predicted maximum density calculated according to maximum density of E. coli grown in 50% of deuterium.
Figure 4.
Maximum growth rate, lag time and maximum density of aged E. coli.
(A) Maximum growth rate of aged E. coli grown in M9 minimal media with ultralow composition of deuterium. * denotes p<0.05. (B) Lag time of aged E. coli grown in M9 minimal media with ultralow composition of deuterium. (C) Maximum density of aged E. coli grown in M9 minimal media with ultralow composition of deuterium. * denotes p<0.05.