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Figure 1.

Vessels 1 (v1) and 2 (v2) are larger than vessel 3 (v3) and 4 (v4).

V1 is an artery, v2 is a vein, v3 and v4 cannot be allocated clearly.

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Figure 1 Expand

Figure 2.

The artery shows a reflection-zone extending from the optic disc to the periphery of the retina.

The reflection of the vein cannot be traced to the periphery. Compared to the venous cross section, the reflection is smaller. The venous vessel wall appears thicker.

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Figure 2 Expand

Figure 3.

The darker vessel is a vein, the brighter an artery.

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Figure 3 Expand

Figure 4.

An example of the difference in size can be observed between vessel 1 and vessel 2.

V1 is an artery, v2 is a vein.

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Figure 5.

Three rings around the optic disc ensure vessel grading at consistent eccentricity for each vessel.

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Figure 6.

Alternate vessels with low variance of thickness and brightness.

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Table 1.

Inter-rater-agreement for 1st and 2nd choice.

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Table 2.

Test-reference agreement of 387 identifiable vessels.

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Table 3.

Sensitivity, specificity and positive predictive value of the test.

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Table 4.

Connection between the test results and the 1st and 2nd choice.

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Table 5.

Correlation between main criteria and test results.

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Table 6.

Distribution of additional criteria, expected frequency means all AC are on an equal level, p-value for the difference between expected and observed frequency.

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Table 7.

Distribution of main criteria, expected frequency means all MC are on an equal level, p-value for the difference between expected and observed frequency.

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Table 8.

Correlation between application of additional criteria and test result based on one main criterion.

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Table 9.

Correlation between application of additional criteria and test results based on zero main criteria.

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Table 10.

Test reference agreement of the second-choice group before and after elimination of AC_1, AC_4 and AC_5.

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Figure 7.

Both the crossing and the outgoing vessels show 90° and 30°-45° angles.

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Table 11.

Quality of potentially identifiable missed vessels applying the 1st or 2nd test option.

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Figure 8.

Workflow for correct vessel labelling in cSLO images.

The different levels of test security are visualized by five colours. MC = main criteria: The central light reflex is wider in arteries and smaller in veins. Arteries are brighter than veins. Arteries are thinner than veins. AC = additional criteria: Arteries never cross arteries and veins never cross veins. Vessels should be seen in the context of the vessel tree.

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Figure 8 Expand

Figure 9.

As an example for vascular diseases cSLO images of four eyes of two patients are shown.

Picture A and B belong to a patient suffering from cerebral vasculitis. Picture C and D arise from a patient who is affected by giant-cell arteriitis. A change of vessel characteristics is not noticeable. Vessel labeling was performed using the new workflow.

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