Table 1.
Sequenom PCR primer sequences used to determine DNA methylation on chromosome 18.
Figure 1.
Comparison of DNA methylation levels at single nucleotide resolution obtained using RRBS and levels measured at the same sites using Sequenom analysis. Four neighbouring CpG sites are shown as an example of concordant results.
Figure 2.
Average DNA methylation in regions annotated as genes and intergenic regions.
Histogram of average DNA methylation calculated for (a) annotated genes and (b) intergenic regions.
Figure 3.
DNA methylation around transcription start sites.
Average DNA methylation 6% for each data point and therefore too small to be represented in the figure.
Figure 4.
CpG content around transcription start sites in the sheep genome (OARv3.1).
CpG content 6(A), and a histogram of gene counts with 0–14% CpG content in 1000 bp around the TSS (B).
Figure 5.
DNA methylation around transcription start sites relative to CpG content and gene expression.
Average DNA methylation of highly expressed vs. repressed genes in three sheep across 6; a) genes with high CpG content, b) low CpG content. Standard error of the difference was less than 1.3% for each data point and therefore too small to be represented in the figure.
Figure 6.
Single nucleotide resolution analysis of DNA methylation.
Histogram of average DNA methylation calculated for all single CpG sites with a minimum read depth coverage of 10.