Figure 1.
Distribution of the samples sequenced in the current study for Tympanocryptis tetraporophora.
Symbols represent different mtDNA clades identified in Fig. 2 and shading represents the current distribution of Tympanocryptis tetraporophora [22]. Map inset shows the Darling Downs, with locations of samples included in our study and the distribution of pre-European (1750) vegetation: tussock grasslands (dark green); brigalow/belah and Callitris forest (light green). Plotted using ArcGIS 10.0 onto “Countries 2006” and NVIS Major Vegetation Groups (Version 4.1) - PRE 1750.
Table 1.
Morphological measurements and meristic character counts for four genetic lineages identified in species tree analyses (Fig. 3).
Figure 2.
Bayesian phylogenetic tree for Tympanocryptis tetraporophora based on ∼1400 bp mitochondrial DNA (ND2).
Samples sequenced in the current study are designated by museum registration numbers and previously published sequences are designated by GENBANK numbers. Figure (A) represents ingroup samples and (B) outgroups. Posterior probabilities are provided on the branches. Symbols on the clades are those from Fig. 1. Large arrow indicated the phylogenetic position of the T. tetraporophora lectotype (NMVD7701).
Table 2.
Uncorrected genetic distance between five well supported mtDNA lineages.
Figure 3.
Bayesian phylogenetic tree for Tympanocryptis tetraporophora based on ∼1200 bp nuclear DNA (RAG1).
Samples sequenced in the current study are designated by museum registration numbers and previously published sequences are designated by GENBANK numbers.
Figure 4.
Gene and species tree phylogenies based on datasets inferred using *Beast: A). mtDNA (ND2); B). nuclear (RAG1); and C). species tree.
Clade posterior probabilities are indicated on branches (*>90%; **>98%). Symbols on the clades are those from Fig. 1.
Figure 5.
Plot of canonical variables 1 and 2 from the discriminant function analysis of morphology showing the relative position for each of the four putative species for (A) males and (B) females.
Ellipses represent 95% confidence ellipses for samples sizes >2.
Figure 6.
Results from the Mitochondrial Tree – Morphological Character Congruence (MTMC), Integrative Taxonomic (ITAX) and Wiens and Penkrot (WP) approaches to species delimitation.
Taxonomies are summarized above each figure by a horizontal multicolored bar, each segment representing a different species. Red line in WP approach represent evidence of gene flow between lineages. Figure template adapted from [5].
Figure 7.
Photographs in life and habitat of Tympanocryptis condaminensis sp. nov.
(a) Kunari Station, Darling Downs; (b) & (c) Bongeen, Darling Downs (photos S. Wilson); and (d) typical habitat - Bongeen, Darling Downs (photo S. Wilson).
Figure 8.
Holotypes of: (a) Tympanocryptis pentalineata sp. nov.; (b) Tympanocryptis condaminensis sp. nov.; and (c) Tympanocryptis wilsoni sp. nov.
Dorsal, ventral and lateral views are shown for each holotype. Details on types series for these species are in Table 3.
Table 3.
Summary of meristic (mm) and mensural data for the types of T. condaminensis sp. nov., T. wilsoni sp. nov., T. penatalineata sp. nov., and T. tetraporophora.
Figure 9.
Photographs in life and habitat of Tympanocryptis wilsoni sp. nov., 40 km west Roma, Darling Downs.
Photos by S. Wilson.
Figure 10.
Photographs in life, specimens before preservation (showing colour and pattern variation) and habitat of Tympanocryptis tetraporophora sensu stricto from Mt Dare, Northern Territory, which is in the topotypic region.
MtDNA phylogeny indicates these individuals are in the same clade as the Lectotype NMVD7701 (Fig. 2a). In image (b) top row are males (x 5) and bottom row are females (x 3). Photos by R. Glor.
Figure 11.
Lectotype of Tympanocryptis tetraporophora (NMVD7701): dorsal and ventral view.