Figure 1.
Percentage composition of amino acids in the phloem sap from pineapple sweet orange: A) Phloem sap collected by centrifugation and derivatized with MTBSTFA, B) Phloem sap collected by EDTA exudation and derivatized with MTBSTFA, C) Phloem sap collected by centrifugation and derivatized with MCF, and D) Phloem sap collected by EDTA exudation and derivatized with MCF.
Figure 2.
Percentage composition of organic acids in the phloem sap from pineapple sweet orange: A) Phloem sap collected by centrifugation and derivatized with TMS, B) Phloem sap collected by EDTA exudation and derivatized with TMS, C) Phloem sap collected by centrifugation and derivatized with MCF, D) Phloem sap collected by EDTA exudation and derivatized with MCF, E) Phloem sap collected by centrifugation and derivatized with MTBSTFA, and F) Phloem sap collected by EDTA exudation and derivatized with MTBSTFA.
Figure 3.
Percentage composition of sugars in the phloem sap from pineapple sweet orange: A) Phloem sap collected by centrifugation and derivatized with TMS and B) Phloem sap collected by EDTA exudation and derivatized with TMS.
Figure 4.
Chemical composition of pineapple sweet orange expressed as percentage composition of the major groups (averages of the different derivatization methods): A) Percentage composition of the phloem sap prepared by centrifugation method and B) Percentage composition of the the phloem sap prepared by EDTA. C) Percentage composition of phloem sap (averages from A and B).
Table 1.
Amino acids and organic acids detected in pineapple sweet orange phloem sap by GC-MS after MCF derivatization (n = 5).
Table 2.
Amino acids and organic acid detected in pineapple sweet orange phloem sap by GC-MS after MTBSTFA derivatization (n = 5).
Table 3.
Sugars and other metabolites detected in phloem sap by GC-MS after TMS derivatization (n = 5).