Figure 1.
Distribution of shatter resistance, as measured with the pendulum test, among DH lines from the BLN2762/Surpass 400 population grown under three environments: experiment 1 (2011, screenhouse, SHT11); experiment 2 (2012, screenhouse, SHT12); and experiment 3 (2012, screenhouse, SHTWW12).
Pair-plots of EBLUPS from DH lines and parental lines showing correlations are presented. Rupture energy (RE) was measured in mJ. Data from experiments SHT11, SHT12 and SHTWW12 were RE, whereas for experiments RELSQ11, RELSQ12 and RELSQWW12 the data were RE (adjusted for pod length) from the same lines.
Table 1.
Significant QTL associated with resistance to pod shatter (pod strength or rupture energy) identified in a doubled haploid population derived from BLN2762/Surpass400
Figure 2.
Box-plot showing variation for resistance to pod shatter in population of DHs from BLN2762/Surpass400, grown under three different environments: Experiment 1, 2010, Birdcage; Experiment 2, 2011, Birdcage; and Experiment 3, 2011, Field conditions).
Figure 3.
QTL detected with linkage (whole average genome interval mapping – WGAIM [DH-QTL], statistical machine learning-SML [DH-QTL], and genome-wide association analysis [GWAS] in Brassica napus germplasm.
Marker sequences were aligned with the sequenced genomes of B. rapa and B. oleracea and their physical positions are shown with dotted lines (Tables S1& S4). Putative candidate genes (marked with red lines) that were localised within the physical map intervals are listed. Only QTL consistent across environments are shown (Table 1): a = Qrps.wwai-A03a and Qrps.wwai-A03b; b = Qrps.wwai-C03; c = Qrps.wwai-A09a, and Qrps.wwai-A09; b and d = Qrps.wwai-C08a, Qrps.wwai-C08b and Qrps.wwai-C08c.
Figure 4.
Anatomical differences among four exemplar haplotypes from the DH population derived from BLN2762/Surpass400.
a = DH line 6668 is a haplotype with Shp(B) and Ind(S) alleles, b = DH line 6823 is a haplotype with Shp(B) and Ind(B) alleles, c = DH line 7128 has Shp(S) and Ind(B) alleles, and d = DH line 7124 has Shp(S) and Ind(S) alleles. Alleles B and S given in parentheses represent the parental donor lines of the DH population. Arrows show marked structural differences among haplotypes.