Table 1.
Baseline characteristics of the study subjects.
Table 2.
Genotype and Allele Frequencies of A1166C variant of the Angiotensin II type 1 receptor gene in the study subjects.
Figure 1.
Intergenotypic (A1166C variant of the Angiotensin II type 1 receptor gene) variations in systolic and diastolic blood pressure in patients with essential hypertension.
Figure 1A represents the scatter plot between Intergenotypic (A1166C variant of the Angiotensin II type 1 receptor gene) variations and systolic blood pressure. Figure 1B shows the scatter plot between Intergenotypic (A1166C variant of the Angiotensin II type 1 receptor gene) variations and diastolic blood pressure. Results were expressed as mean ± SD.
Figure 2.
Semi-quantitative analysis of Angiotensin II type 1 receptor gene expression.
Figure 2A represents the semi-quantitative analysis of AT1R gene. Figure 2B illustrates the relative AT1R gene expression in terms of integrated densitometric value (IDV) of patients (2.66±0.62) and normal healthy controls (2.15±0.28). Results were expressed as average densitometric ratio (AT1R to 18S rRNA gene) in patients and controls group ± SD. p<0.0001.
Figure 3.
delta-CT values for study subjects.
Figure 3 represents the scatter plot of delta-CT values for patients and controls group. The higher delta-CT value represents the lower expression of gene at mRNA level.
Table 3.
Fold change expression and Quantitative PCR results of Angiotensin II type 1 receptor gene.
Figure 4.
Intergenotypic (A1166C variant of the Angiotensin II type 1 receptor gene) variations in gene expression (delta-CT values) in study subjects.
Figure 4 represents the Intergenotypic variations in gene expression (delta-CT values) in study subjects. Results were expressed as mean ± SD. *p<0.05 is considered to be significant.
Table 4.
Intergenotypic (A1166C variant of the Angiotensin II type 1 receptor gene) variations in gene expression (delta-CT values) in study subjects.
Figure 5.
Western blot analysis of Angiotensin II type 1 receptor protein expression.
Figure 5A represents the western blots of AT1R and β-actin protein in controls and patients respectively. Figure 5B shows the relative AT1R protein expression in terms of integrated densitometric value (IDV) of patients (1.49±0.27) and normal healthy controls (0.80±0.24). Results were expressed as average densitometric ratio (AT1R to β-actin protein) in patients and controls group ± SD. p = 0.0001.