Table 1.
Transcriptome statistics.
Figure 1.
Comparison of Strombidium rassoulzadegani and Strombidinopsis sp. transcriptomes.
A-B Frequency distribution of GC content in ciliate transcripts, algal food transcripts, and putative food transcripts filtered from ciliate data. C- Venn diagram of all-against-all BLASTP hits between ciliates. D- Venn diagram of orthologous and paralogous amino acid sequence groups in ciliates.
Figure 2.
BLASTP hit distribution in ciliate transcripts.
A- Proportion of amino acid sequences. B- Proportion of Illumina reads realigned to the corresponding coding regions.
Figure 3.
Gene Ontology terms distribution in ciliate transcripts.
Figure 4.
Hypothetical pathways for reactive oxygen species (ROS) detoxification.
Pathways evidenced in both ciliates (white background) or only in Strombidium rassoulzadegani (yellow background) are shown. In red, ROS (O2- = superoxide radical; H2O2 = hydrogen peroxide); in dark blue, antioxidant enzymes (SOD = superoxide dismutase; Cat = catalase; APX = ascorbate peroxidase; GPX = glutathione peroxidase; TPX = thioredoxin peroxidase); in light blue, enzymes involved in metabolism of non-enzymatic antioxidants (Nec3 = bifunctional monodehydroascorbate reductase/carbonic anhydrase nectarin-3-like; TRed = thioredoxin reductase; GRed = glutathione reductase; GSyn = GSH synthetase; GCLig = Glutamate-cysteine ligase); in green, non-enzymatic antioxidants (AsA = ascorbic acid; GSH = glutathione; Trx = thioredoxin). MDAs = monodehydroascorbate; GSSG = glutathione disulfide; TrxS2 = thioredoxin disulfide; NAD(P)+ = nicotinamide adenine dinucleotide (phosphate). Based on literature [61], [63], [75].