Figure 1.
Schematic representation of a longitudinal cross section through a Brachypodium embryo with specific organ tissues marked.
Figure 2.
Seeds of Brachypodium with a ‘matured’ (A), ‘dry’ (B) and ‘germinating’ (C) embryo. Bar: 1 mm.
Figure 3.
Longitudinal cross sections through the whole ‘matured’ (A), ‘dry’ (B) and ‘germinating’ (C) Brachypodium embryo. Bar: 0.5 mm.
Figure 4.
Starch accumulation in ‘matured’ (A–D), ‘dry’ (E–H) and ‘germinating’ (I–L) Brachypodium embryos detected by PAS reaction.
Cross sections through the scutellum (A, E, I), coleoptile and SAM with leaf primordia (B, F, J), RAM (C, G, K), the root cap and coleorhiza (D, H, L). Bar: 50 µm.
Figure 5.
The immunodetection of H4K5ac in ‘matured’ (A–D), ‘dry’ (E–H) and ‘germinating’ (I–L) Brachypodium embryos.
Cross sections through the scutellum (A, I), the scutellum, coleoptile and leaf primordia (E), the SAM with leaf primordia (B, F, J), the RAM (C, G, K), the distal part of RAM, the root cap and coleorhiza (D, H) and the coleorhiza (L). Bar: 50 µm. Enlargements of selected cross sections are provided (Figure S1).
Figure 6.
The immunodetection of H3K4me1 in ‘matured’ (A–C), ‘dry’ (D–G) and ‘germinating’ (H–K) Brachypodium embryos.
Cross sections through the scutellum (A, D, H), the coleoptile and SAM with leaf primordia (B), the coleoptile and leaf primordia (E, I), the SAM (J), the RAM, the root cap and coleorhiza (C, K), RAM (F), the distal part of RAM and the coleorhiza (G). Bar: 50 µm.
Figure 7.
The immunodetection of H3K4me2 in ‘matured’ (A–C), ‘dry’ (D–G) and ‘germinating’ (H–K) Brachypodium embryos.
Cross sections through the scutellum (A, E, I), the coleoptile and leaf primordia (B, F, J), the SAM with leaf primordia (G, K), the proximal part of RAM (C), the RAM, root cap and coleorhiza (D, H, L). Bar: 50 µm. Enlargements of selected cross sections are provided (Figure S1).
Table 1.
Relative intensity of the immunosignals in Brachypodium embryos.