Table 1.
Half-site organization for TRE constructs.
Figure 1.
Transcriptional activity determined by luciferase reporter assay for DR4 (A), IR0 (B) and ER6 (C). COS-7 cells were co-transfected with a TRα and/or RXRα expression vectors.
Cells were treated with 5 nM T3 and/or 1 µM 9cRA alongside untreated controls. Firefly luciferase expression was normalized to renilla luciferase expression. Experiments were run in triplicate and repeated at least 3 times. Data for each condition are shown separately since each experiment is paired and was run independently. Data are presented as means ± standard error of the mean. Asterisks (*) denote a significant difference, p≤0.05, determined by a two-tailed, paired Student's t-test.
Figure 2.
Antagonistic effects of 1–850 determined by luciferase reporter assay for DR4 (A) and ER6 (B).
COS-7 cells were co-transfected with a TRα or TRα+RXRα expression vectors. Cells were treated with or without 5 nM T3, or with T3 in the presence of 10 µM 1–850. Firefly luciferase expression was normalized to renilla luciferase expression. Experiments were run in triplicate and repeated at least 3 times. Data are presented as means ± standard error of the mean. Asterisks (*) denote a significant difference, p≤0.05, determined by one way ANOVA followed by Tukey's HSD test; (ns) denote no significant difference, p≥0.05.