Figure 1.
Results of wing-specific RNAi expression and the most typical manifestations of resulting blistered and “burned” wings.
(A) General statistics of the RNAi screening. Blistered and “burned” wings were the most frequent phenotypes. (B–G) Examples of blistered wings with the blister occupying the whole wing (B, RNAi-Fas1) or the central position in the wing (C, RNAi-rok), or blisters accompanied with other wing defects such as narrow wings (D, RNAi-CG10754), “horned” wings (E, RNAi-RnrL), “swarovski” wings (F, RNAi-Hsp83), or “stump” wings (G, RNAi-U2af50). (H–J) Examples of “burned” wings. This phenotype may start as blistered wings in freshly eclosed flies (H) and with age develop into necrotic wings (I, RNAi-Wg), or reveal “burned” wings throughout the adult life (J, RNAi-mys).
Figure 2.
Semantic clustering of the GO terms enriched in the blister-causing group, compared to the annotations of all screened genes.
Enriched GO terms are shown as nodes (“biological processes” as octagons and “cellular components” as circles), and the top 3% of the strongest GO term pairwise similarities are designated as edges in the graph. The node radius relates to the generality of the terms, where smaller nodes imply more specific terms; the supplied p-values/enrichments are shown using color shading, where more saturated color of the node implies more over-represented GO terms. The semantic similarity between two GO terms is shown by the thickness of the edges, where thicker edge implies more semantically similar GO terms connected by this edge.
Figure 3.
Each gene is an independent node, with edges between them being interactions of the genetic (cyan) or physical (red) nature, or being inferred from the physical interactions among their human orthologues (lilac). Nodes are color-coded and grouped into functional clusters according to their annotation terms; interactions within each functional cluster are shown by bold edges. Grey nodes represent genes which failed to be clustered. Genes not interconnected into the large blisterome network are grouped below it; however, some of them are color-coded, because they still belong to functional clusters of the major network. Nodes are given as circles if coming from our RNAi screening only, rectangles or triangles if coming solely from previous loss- or gain-of-function analysis, or other symbols if coming from both our and previous analysis; complete description of the node shape coding is given in the lower left corner of the Figure.
Figure 4.
The network composed of human orthologues of the Drosophila blisterome components.
Physical interactions are shown in grey, phenotypic – in blue. Disease-related genes are marked by red crosses. Nodes are color-coded and grouped into separate functional clusters according to their annotation terms; interactions within each functional cluster are shown by bold edges. Grey nodes represent genes which are not enriched by any term. However, some of these nodes were placed close to existing clusters if most of their connections were with its members; the remaining nodes with promiscuous interactions were grouped in the center of the network. To reduce Figure complexity isolated nodes were removed leaving only the highly interconnected ones.
Figure 5.
Human blisterome orthologues linked to diseases and disorders.
(A) Distribution of the disease-related genes among major systems of organs affected by specific maladies associated with these genes. (B) Distribution of the disease-related genes by the number of systems they affect (left bar) (see Table S9) and diseases they cause (right bar) (see Table S8). The quantity of assigned genes is indicated by numbers on the graphs on both panels (A) and (B).