Table 1.
Association between BANCR and clinicopathological parameters of patients.
Figure 1.
Increased expression of BANCR in both malignant melanoma tissues and cell lines.
(A) Increased BANCR expression in malignant melanoma tissues compared to control skin tissues. (B) BANCR expression increased with clinical stages of malignant melanoma. (C) Significant high expression of BANCR in five melanoma cell lines in comparison with control skin tissues pooled from 3 controls with melanocytic nevus. (** P<0.01).
Figure 2.
Effects of BANCR on proliferation melanoma cells.
(A) Expression of BANCR was significantly silenced by transfecting sk-mel-5 cells with shRNA. Loss of BANCR expression significantly inhibited (B) proliferation of sk-mel-5 cells, tumor growth, including (C) tumor weight and (D) volume in nude mice. Loss of BANCR expression significantly inhibited proliferation of UACC903 (E) and CHL-1 (F) cells, (**P<0.01, Figure is representative of 3 experiments with similar results.)
Figure 3.
The activities of ERK1/2, Raf-1 and JNK in BANCR silencing sk-mel-5 cells were significantly repressed.
Expressions of ERK1/1, Raf-1, p38 and JNK in BANCR silencing sk-mel-5 cells were detected by western blot. Loss of BANCR induced the inactivation of (A) ERK1/2, (B) JNK and (C) the upstream molecule of ERK1/2, Raf-1. (D) However, no activation was observed in p38 MAPK. GAPDH expression was used to normalize for equal loading. Figure is representative of 3 experiments with similar results.
Figure 4.
Inactivation of ERK1/2 and JNK participated in BANCR-regulated proliferation.
Cells transfected with sh-BANCR or BANCR plasmid were treated with U0126 or SP600125 respectively. Western blot was performed to detect total and phosphorylation changes of ERK1/2 and JNK. U0126 or SP600125 induced significant inactivation of ERK1/2 (A) and JNK (C). Cell proliferation decreased significantly after 72 h (B, D). Note the notable effects induced by combined treatment with shRNA and inhibitors. BANCR activated ERK1/2 (E) and JNK (G) pathways. Inactivation of ERK1/2 and JNK were rescued by overexpression of BANCR. Inhibitory proliferation induced by ERK1/2 and JNK inactivation was ameliorated by BANCR (F, H). Figure is representative of 3 experiments with similar results.
Figure 5.
Kaplan-Meier survival curves of malignant melanoma patients relating to the status of BANCR expression.