Figure 1.
Erythroid differentiation of C19 iPSC CD34+ cells.
C19 and adult peripheral blood [PB] CD34+ cells were incubated for up to 21 days in our three-stage erythroid culture system. (A) Morphological analysis of cells stained with May-Grundwal Giemsa reagent on day 7, 13 and 19 in culture. Arrows, white proerythroblasts, blue basophillic erythroblasts, red polychromatic erythroblasts, black orthochromatic erythroblasts. (B) Western blot of iPSC and PB erythroid cells at day 19 in culture, and undifferentiated [undif] iPSCs, probed with antibodies to α-,β- and γ-globin, GPA and Band 3. Antibodies to actin were used as a protein loading control. Numbers on left are size markers. (C) iPSC erythroid cells at day 19 in culture probed with antibodies to GPA, Rh, GLUT1 and Protein 4.2, followed by compatible secondary antibodies with Alexa Fluor 488 (green) or Alexa Fluor 635 phalloidin (red). Arrows indicate reticulocytes. (D) Erythroid cells differentiated from C19 iPSC and PB progenitors were incubated with Alexa Fluor 635 phalloidin conjugated actin antibody (red). Arrows indicate contractile actin rings. Nuclear DNA was stained with blue-fluorescent DAPI. Images were obtained using a Leica SP5 confocal microscope. Scale bars 10 µm.
Figure 2.
Levels of selected erythroid proteins in erythroid cells differentiated from iPSC, PB and CB.
Western blot of day 19 adult [PB], cord blood [CB] and C19 iPSC erythroid cell lysate (30 µg) probed with antibodies to Duffy and CD44, and day 8 lysate probed with antibodies to adducing α, catenin α (CTNNA1) and Tubulin β. Antibodies to actin were used as a protein loading control at both time points.
Table 1.
Hallmark red cell proteins in erythroid cells differentiated from C19 iPSCs analysed by nanoLC-MS/MS.
Figure 3.
Difference in the level of globin subunits between erythroid cells differentiated in vitro from adult peripheral blood (PB) CD34+ cells, and from C19, OCE1 and OPM2 CD34+ cells.
PB, C19, OCE1 and OPM2 erythroid cells at day 8 in culture were lysed, proteins subjected to trypsin digest and resultant peptides labeled with isobaric tags for nanoLC-MS/MS based quantitation and comparison. Y-axis represents the fold difference in protein level between erythroid cells differentiated from each iPSC line and adult PB progenitors.
Figure 4.
Difference in the level of RBC hallmark and cytoskeleton proteins between erythroid cells differentiated in vitro from adult peripheral blood (PB) CD34+ cells, and from C19, OCE1 and OPM2 CD34+ cells.
Erythroid cells differentiated from PB progenitors, C19, OCE1 and OPM2 iPSCs at day 8 in culture were lysed, proteins subjected to trypsin digest and resultant peptides labeled with isobaric tags for nanoLC-MS/MS based quantitation and comparison. Y-axis represents the fold difference in protein level between erythroid cells differentiated from each iPSC line and adult PB progenitors.
Figure 5.
Expression of BCL11A and KLF1 in erythroid cells differentiated in vitro from C19 iPSC, cord blood (CB) and adult peripheral blood (PB) CD34+ cells.
Western blot of total protein from undifferentiated iPSCs (undif iPSC), and erythroid cells differentiated from iPSCs, CB and PB CD34+ cells at day 8 in culture probed with BCL11A antibody, stripped and re-probed with KLF1 antibody and stripped and re-probed with an antibody to tubulin as a protein loading control. * indicates non-specific band detected by antibody.
Figure 6.
Difference in the level of proteins between adult (PB) and iPSC erythroid cells, compared to that between cord blood (CB) and iPSC erythroid cells.
PB, C19, OCE1 and OPM2 erythroid cells at day 8 in culture were lysed, proteins subjected to trypsin digest and resultant peptides labeled with isobaric tags for nanoLC-MS/MS based quantitation and comparison. The levels of 50 proteins with the greatest differential expression between (A) PB and C19, OCE1 or OPM2 erythroid cells and (B) CB and C19, OCE1 or OPM2 erythroid cells were all compared between PB and the 3 iPSC lines and CB and the 3 iPSC lines. Each point on the x-axis represents a unique protein. Y-axis represents the fold difference in protein level between erythroid cells differentiated from PB or CB and the iPSC line.