Figure 1.
Schematic representation of the biosensor.
Schematic representation of the biosensor for the detection of pLDH based on the surfactant-induced aggregation of AuNPs.
Figure 2.
Optimization of CTAB and pL1 aptamer concentrations.
(A) The effect of the concentration of CTAB on pLDH detection. The inset figure represents the visual color change of the AuNPs at each concentration level. (B) The effect of the concentration of the pL1 aptamer on pLDH detection. The inset figure shows the visual color change of the AuNPs at each concentration level. (A and B) Points and error bars represents the means and standard deviations, respectively, of three repeated measurements.
Figure 3.
Colorimetric detection of recombinant PvLDH.
(A) The calibration curve of the sensing solutions containing varying concentrations of PvLDH. Points and error bars represents the means and standard deviations, respectively, of three repeated measurements. The inset figures represent the visual color changes of the AuNP solutions. (B) The absorbance spectra for the detection of PvLDH.
Figure 4.
Detection of PvLDH in the human serum sample.
(A) The calibration curve of the sensing solutions containing varying concentrations of PvLDH in the serum sample. Points and error bars represents the means and standard deviations, respectively, of three repeated measurements. (B) The TEM image of the AuNP solution containing the serum sample. (C) The TEM image of the AuNP solution containing 10 nM of PvLDH in the serum sample.