Table 1.
Development of embryos derived from sperm treated with 1500 µM H2O2.
Figure 1.
Effect of treating sperm with 1500 µM H2O2 on subsequent (A) blastocyst cell number, (B) trophectoderm cell number, (C) inner cell mass (ICM) cell number, (D) percentage (%) of ICM/total cell number.
Control (n = 84 embryos), H2O2 (n = 79 embryos). Values are mean ± SEM.* Indicates significantly different from control (P<0.05) ** indicates significantly different from control (P<0.01).
Table 2.
Implantation and placental and fetal parameters at day 18 of gestation.
Figure 2.
Effect of treatment of sperm with 1500 µM H2O2 on female offspring weight gain and glucose tolerance.
(A) pre-weaning weight, (B) post weaning weight, (C) glucose tolerance (AUC) Control female (n = 7 from 6 litters), H2O2 female (n = 9 from 5 litters) representative of 15 stud CBAF1 males. Values are mean ± SEM.* Indicates significantly different from control (P<0.05).
Table 3.
Post mortem female offspring body composition and fasting blood metabolites sired by control sperm or sperm treated with 1500 µM H2O2 at 17 weeks of age.
Figure 3.
Effect of treatment of sperm with 1500 µM H2O2 on male offspring weight gain and glucose tolerance.
(A) pre-weaning weight, (B) post weaning weight, (C) glucose tolerance (AUC). Control male (n = 14 mice from 6 litters), H2O2 male (n = 7 mice from 5 litters) representative of 15 stud CBAF1 males. Values are mean ± SEM.* Indicates significantly different from control (P<0.05).
Table 4.
Post mortem male offspring body composition and fasting blood metabolites sired by control sperm or sperm treated with 1500 µM H2O2 at 17 weeks of age.