Figure 1.
The anneal curve reactions of different ostrich meat reference DNA.
The products showed a melting temperature of 86.5°C (±0.25).
Table 1.
Oligonucleotide primers sequences used for LAMP assay.
Table 2.
Analytical sensitivity of the LAMP assay using serial dilution of ostrich DNA.
Figure 2.
Box plots of detection time of LAMP assay with a detection limit up to 0.01% of ostrich.
The Box plots was generated by using six replecates for each concentration.
Figure 3.
Amplification signal of ostrich meat DNA using the direct swab and HYPLEX buffer test method.
The red curve is the signal of ostrich DNA isolated with DNeasy tissue Isolation Kit (detection time of 6∶18 min). The other positive curves in the oval shap are the direct swab samples with a detection time of 11∶03–12∶18 min. The pink curve is the negative control (see table 3).
Table 3.
Time of detection and annealing temperatures of a dry swab and HYPLEX LPTV buffer test method.