Table 1.
Association between LASP-1 expression and clinical characteristics in ccRCC patients.
Figure 1.
LIM and SH3 protein 1 (LASP-1) expression in clear cell renal cell cancer (ccRCC) tissues and cell lines.
LASP-1 protein expression in paraffin-embedded ccRCC tissues (A) and adjacent nontumorous tissues (B) using immunohistochemistry (magnification, 100×), in which positive LASP-1 immunostaining showed brown color. Wilcoxon analysis demonstrated that tumor tissues showed significantly higher LASP-1 expression than nontumorous tissues (C, n = 216). Western blot (D) and real-time PCR (E, n = 20) analyses confirmed the findings in immunohistochemistry analysis. Western blot analysis also showed differential LASP-1 expression in human embrynal kidney cells (HEK-293) and ccRCC cell lines (F). T refers to tumor tissues, whereas P refers to peritumor (nontumorous) tissues in panel D.
Figure 2.
LIM and SH3 protein 1 (LASP-1) overexpression in clear cell renal cell cancer (ccRCC) is associated with poor overall survival (A) and recurrence free survival (B).
Table 2.
Univariate Cox regression analysis of the prognostic factors for ccRCC.
Table 3.
Multivariate Cox regression analysis of the prognostic factors for ccRCC.
Figure 3.
LASP-1 silencing inhibited RCC cell migration in vitro.
Western blotting was used to verify knock-down of LASP-1 expression in 786–0 cells by siRNA transfection (A). An unpaired t-test was used to assess differences in the number of invaded cells per field between the 786–0 and si786–0 cell lines (B). Transwell results for the 786–0 and si786–0 cell lines are shown (C).