Figure 1.
Camera view experimental set-up of (A) potential analyzer (CV-50W) is connected with bio-chip onto electronic board via electronic-pins, (B) magnified view of controlled and fabricated biochip, (C) Three-electrodes system of WE, CE, and RE (perpendicular onto biochip) assembled onto bio-chip.
The WE is directly immersed into the electrolyte-drop perpendicularly.
Figure 2.
Schematic diagram of (A) top camera-view, (B) magnified view of sensing area, (C) total cross-sectional view, and (D) cross-section of sensing area of bi-chips.
Figure 3.
Schematic representation of fabrication procedure for cholesterol biosensor using tiny bio-chips.
Sensing area of biochip: 0.0805 cm2; TGA: 10.0 mM/ethanol; EDC: 10.0 mM; ChOx: 10.0 mM.
Figure 4.
(A) CVs of 5.0 mM K3Fe(CN)6/PBS (0.1 M) for bare (black curve) and TGA-SAM (blue curve) modified electrodes; (B) CVs recorded in 0.1 M phosphate buffer solution of bare chip (black curve), ChOx modified chip (blue curve), and in presence of 0.1 mM cholesterol (green curve) solution; (C) pH effect of Au/TGA/ChOx electrode in 0.1 mM cholesterol solution with the bio-chip.
Scan rate: 0.1 V/s, RE: Ag/AgCl (saturated KCl), Supporting electrolytes: 0.1 M phosphate buffer solution.
Figure 5.
Electrochemical sensor responses of (A) variation of cholesterol concentrations (1.0 nM to 100.0 mM), (B) calibration curve, and (C) linearity of developed on bio-chip at room conditions.
Scan rate: 0.1 V/s; Concentration range of cholesterol: 1.0 nM to 100.0 mM; Method: CV; Reference electrode: Ag/AgCl (sat. KCl).
Table 1.
Evaluation of the Au/TGA/ChOx-biochip cholesterol sensor performances compared with various enzyme-material conjugated sensors.