Table 1.
Summary of sequencing results and OTU (Operational Taxonomic Unit) richness of Porites astreoides microbiomes. Sample names designated “PN” were collected from visually nonsymptomatic corals and those designated “PL” were collected from lesioned corals.
Figure 1.
Gross morphology of lesions on Porites astreoides.
P. astreoides colonies were photographed in July (A, C, D) and November (B) of 2012. The same colony is shown in (A) and (B). (C) and (D) are close-up photographs of the lesion. Note yellow band (a hypothetical biofilm) at the boundary of the denuded coral skeleton and bleached coral tissue. Polyps ahead of the yellow band are bleached, but do not appear retracted. Photographs by Abby Wood.
Figure 2.
Bacterial community composition in Porites astreoides microbiomes.
Samples are grouped by disease state (lesioned, designated “PL” (highlighted in grey), or nonsymptomatic, designated “PN” (highlighted in green)) and by collection month. Samples with the suffix “S” were collected in July 2012, all other samples were collected in September 2012.
Figure 3.
Comparison of surface microbiomes of Porites astreoides with and without visible lesions.
Panel A: UPGMA (Unweighted Pair Group Method with Arithmetic mean) clustering of Porites surface microbiomes based on the Morisita-Horn beta diversity of V4 region of 16S rRNA genes revealed two distinct clusters of samples (designated “Cluster 1” and “Cluster 2”). The scale bar represents the beta diversity distance. Samples collected from visibly lesioned corals are labeled with a black diamond and are designated “PL”; samples from nonsymptomatic corals are designated “PN”. Samples collected in July are designated with the suffix “S”, all other samples were collected in September. Panel B: Relative abundance of dominant genera (comprising ≥1% of reads in any individual microbiome) in each Porites microbiome based on16S rRNA gene Operational Taxonomic Units.
Table 2.
Summary of Simper analysis, showing the contribution of major genera (contributing ≥ 1%) to the dissimilarity between nonsymptomatic and lesioned coral microbiomes.
Figure 4.
Positive and negative species correlations in Porites astreoides microbiomes.
Significant correlations (p≤0.05) between OTUs (Operational Taxonomic Units) in Porites astreoides microbiomes, as determined by variance of log-ratios with 100 iterations. Node size is indicative of the relative abundance of the OTU in the entire dataset.