Figure 1.
The virulence of the human Shigella strain ZD02 using the Sereny test and the HeLa cell invasiveness test.
(A) Sereny test. Guinea pigs were infected with Shigella strain ZD02 via the cornea and conjunctiva. Corneal and conjunctiva inflammation were seen in infected eyes at 18 h post-inoculation (A1). Corneas and conjunctiva is normal in uninfected control eyes (A2). (B) HeLa cells infected with the Shigella strain ZD02. Transmission electron microscopy was used to detect the internalized bacteria within HeLa cells. Representative pictures are shown. The internalized bacteria (arrowheads) were detected at 2 h post-inoculation (B1). Control HeLa cells without infection (B2). Scale bars = 1 µm.
Figure 2.
Clinical responses of chickens after intraperitoneal injection.
Each bar denotes number of the clinical response/total in each experimental group after 12 h of intraperitoneal injection with Shigella strain ZD02.
Figure 3.
Histopathology of the intestine of one-day-old SPF chickens infected with the Shigella strain ZD02 via ligated intestinal loop.
The duodenum, jejunum, ileum, and cecum from Shigella-infected chickens showed congestion, edema, and neutrophil infiltration (A–E, respectively). No significant pathological changes were noticed in the uninfected controls (F–J, respectively). The sections were stained with hematoxylin and eosin. Representative pictures are shown. Scale bars = 50 µm.
Figure 4.
Immunohistochemistry of the jejunum of one-day-old SPF chickens infected with the Shigella strain ZD02 via ligated intestinal loop.
Bacterial invasion (brown staining) was noticed in the epithelial cells 6 h (A) and 8 h (B) post-inoculation. The intestinal villus detached and the bacteria (brown staining) were released from the lysed epithelial cells (C). Intestinal villus of uninfected chicken was shown as control (D). Scale bars = 20 µm.
Figure 5.
Transmission electron microscopy of the jejunum of one-day-old SPF chickens infected with the Shigella strain ZD02 via ligated intestinal loop.
Shigella (arrowheads) were found near the epithelial cells at 2 h post-inoculation (A. Scale bar = 1 µm). Internalized bacteria (arrowheads) in the epithelial cells were found at 6 h (B. Scale bar = 5 µm) and 8 h (C. Scale bar = 5 µm). The epithelial cells started to lyse and release bacteria (arrowheads) at 12 h (D. Scale bar = 5 µm).
Figure 6.
Immunohistochemistry of the primary chicken intestinal epithelial cells infected with the Shigella strain ZD02.
Uninternalized bacteria (brown staining) were seen near the epithelial cells at 1 h post-inoculation (A). Internalized bacteria (brown staining) were found at 2 h (B) and 3 h (C). The lysed epithelial cells released bacteria (brown staining) at 4 h (D). Uninfected epithelial cells were shown as control (E). Scale bars = 20 µm.
Figure 7.
Transmission electron microscopy of the primary chicken intestinal epithelial cells infected with the Shigella strain ZD02.
Uninternalized bacteria were seen near the epithelial cells at 1-inoculation (A, B. Scale bars = 2 µm). The internalized bacteria were encapsulated with phagosome-like membranes (C, D. Scale bars = 1 µm).
Figure 8.
Scanning electron microscopy of the primary chicken intestinal epithelial cells infected with the Shigella strain ZD02.
Uninternalized bacteria (arrowhead) were seen near the epithelial cells 1 h post-inoculation (A. Scale bar = 10 µm). Bacteria (arrowheads) attached to the surfaces of the epithelial cells at 1 h (B. Scale bar = 3 µm). Bacteria (arrowheads) were being internalized by the epithelial cells at 2 h (C. Scale bar = 3 µm).