Table 1.
Mapped wheat EST markers and ortholgous gene pairs among Brachypodium, rice and sorghum
Table 2.
EST-STS, EST-SSR and SSR markers linked to powdery mildew resistance gene MlIW172
Table 3.
Genetic analysis of the powdery mildew resistance gene MlIW172
Figure 1.
Genetic and comparative genomics linkage map of powdery mildew resistance gene MlIW172 derived from wild emmer.
A: MlIW172 physical bin map. MlIW172 was mapped to the distal bin 7AL16-0.86-0.90. B: Preliminary MlIW172 genetic map on wheat chromosome arm 7AL with genetic distances in cM shown on the left, markers shown on the right. C: MlIW172 high-resolution genetic map on wheat 7AL arm with genetic distances in cM shown on the left, EST-STS, EST-SSR and SSR markers shown on the right. Molecular markers that were previously assigned to the 7A wheat deletion bin map (A) are connected to the physical map with solid lines. The MlIW172 locus is in red and underlined. The markers which served as anchors, establishing colinearity between the MlIW172 genetic map and the sequences of Brachypodium, rice and sorghum, are connected to the Brachypodium gene with solid lines. D: The MlIW172 orthologous genomic region on Brachypodium chromosome 1 (150kb) with orthologous genes shown on the right. The four genes in green represent the RGA cluster. E: The MlIW172 orthologous genomic region on rice chromosome 6 (85.8kb) with orthologous genes shown on the right. F: The MlIW172 orthologous genomic region on sorghum chromosome 10 (138.9kb) with orthologous genes shown on the right.
Figure 2.
Physical map of the BAC contigs and scaffolds flanking the MlIW172 locus anchored to the high-resolution genetic map.
The approximate physical locations of all the newly designed markers are given on the BAC contigs or scaffolds.
Figure 3.
PCR amplification patterns of the markers WGGC4656, WGGC4657, WGGC4658, WGGC4659, WGGC4660, WGGC4661, WGGC4662, WGGC4663, WGGC4664, and WGGC4665 in 8% non-denatured polyacrylamide gels.
M: 2kb DNA marker. Lanes 1 and 2 are IW172 and Mo75, respectively, lanes 3–6 represent homozygous resistant plants, lanes 7–10 represent homozygous susceptible plants, and lanes 11–14 represent heterozygous resistant plants.
Figure 4.
Integrative views of the MlIW172 gene loci with other previously published Pm genes on chromosome arm 7AL.
The loci can be classified into three groups (Group I; Group II; Group III) based on their order and genetic distance to markers Xwmc525 and Xgwm344.