Table 1.
Growth rate, photosynthetic parameters and the macromolecular composition of BL and RL light acclimated P. tricornutum cultures.
Figure 1.
Long-term acclimation to light quality shifts.
The acclimation process of P. tricornutum cultures was observed for 6 days after the light quality shift from BL to RL and RL to BL. Shown are (A) estimated gross oxygen evolution rates at growth light conditions, (B) growth rate per day, (C) maximum values of non-photochemical quenching and (D) concentration of the xanthophyll cycle pigment Ddx at growth light conditions. The NPQ values were measured at illumination with saturating light intensities, while all other values are measured at growth light conditions. The asterisks depict the p-values of the respective data point compared to the steady state (BL to RL shift is compared to RL steady state, RL to BL shift is compared to BL steady state). n = 4–5. The exact values are specified in Table S1.
Figure 2.
Fast fluorescence induction kinetics.
The changes of the J Level of fast fluorescence induction kinetics (FJ′ FM′−1) were recorded during the light quality changes. (A) RL to BL shift of a RL adapted culture (0–40 min) followed by a light shift back to RL (60–100 min) (B) BL to RL shift of a BL adapted culture (0–40 min) followed by a light shift back to BL (60–100 min). The horizontal lines show the J level of BL (dotted) and RL (dashed) adapted cultures. The asterisks depict the p-values of the respective data point compared to t0 min (0–40 min) or to t60 min (60–100 min). n = 3. The exact values are specified in Table S2.
Figure 3.
Short-term acclimation to light quality shifts.
The changes in (A) the Chl a concentration and (B) ΦPSII of P. tricornutum cultures were recorded for 10 h after the light quality shift at t0 from BL to RL and RL to BL. Additionally, the time in the light phase is given above the plot. The asterisks depict the p-values of the respective data point compared to the steady state the cultures were shifted from at the same time point (Chl a) or the daily mean value when this was constant (ΦPSII). n = 4–8 for the Chl a concentrations and n = 3–4 for FV′ FM′−1. The exact values are specified in Table S3.
Figure 4.
Carbohydrate and protein levels.
The carbohydrate and protein levels of P. tricornutum cultures during the course of the day were determined for cultures pre-acclimated to (A) BL and (C) RL as well as (B) the changes after a shift from BL to RL and (D) RL to BL. Shown are the relative protein and carbohydrate contents in relation to the values measured at t0. The asterisks depict the p-values of the respective data point compared to t0 for pre-acclimated cultures (A, C) or compared to the steady state the cultures were shifted from at the same time point (B, D). n = 3–6. The exact values are specified in Table S4.
Figure 5.
The relative partitioning of C (carbon) into carbohydrates, proteins and lipids was calculated for the 2 h following the light quality changes. Data were calculated on the basis of the FTIR spectra and the net rate of carbon assimilation and, therefore, add up to 100% net carbon assimilation (carbohydrate +protein + lipid bar). Values below zero mean a net loss. Shown are the first 2 h after the light quality change from RL to BL and vice versa. n = 3–6. The exact values are specified in Table S5.
Figure 6.
The changes in the content of metabolites per cell after a light quality change compared to the pre-acclimated P. tricornutum cultures were determined for 4 time points. The asterisks depict the p-values of the respective data point compared to t0. Additionally ‘.’ marks p<0.1. Significant changes that were lower than 10% (marked grey) were omitted. n = 3.
Figure 7.
Changes in the metabolic ratios.
All possible metabolite ratios (e.g. glucose-6P to fructose-6P) of the measured amino acids (black) or of the metabolites of the central carbon metabolism (grey) were calculated for each time point. The figure shows the rate of significant changes of these ratios between the time points given under each bar (e.g. 10% means that 10% of all metabolite ratios between these time points change significantly, p<0.05). This acts as a value for metabolome reorganization. Given are the relevant time points for the (A) RL to BL shift and (B) the BL to RL shift. The left hand bar is similar for (A) and (B) and compares the RL and BL pre-acclimated cultures. The bars in the middle illustrate the changes between shifted cultures to the light quality they were shifted from. The right hand bar compares the shifted cultures at 1 d after the shift to the light quality they were shifted to.