Figure 1.
Overview of sample preparation and processing procedures.
(A) Sample processing workflow showing different steps of enrichment and identification. (i) The blood sample is collected; (ii) Plasma is separated using standard centrifugation (1500×g for 10 min) and stored at −80 degree Celsius for DNA analysis. (iii) RBCs are lysed using ammonium chloride and (iv) sample is processed through multiplexed spiral chip within 10 min. (v) The isolated CTCs are available for immunostaining using standard markers or FISH (fluorescence in situ hybridization). DNA or RNA can be extracted from the CTCs and subjected to next-generation sequencing and q-PCR. Viable cells can be released and propagated in cell culture for various applications including cancer stem cell (CSC) study or drug discovery. (B) Illustration of the design of a multiplexed device (left) and optical image of an actual multiplexed spiral microfluidic device (middle) for capturing CTCs with two inlets and two outlets. Blood sample and sheath fluid are pumped through the device using two separate syringe pumps. Under the influence of inertial lift and Dean drag forces in the fluid flow, CTCs focus near microchannel inner wall (Region A-A) while WBCs and Platelets goes through one Dean cycle and migrate back towards the outer wall (Region B-B), thus achieving separation.
Figure 2.
Enumeration of CTC from cancer patients.
(A) Immunofluorescence staining of isolated CTCs. CTCs (marked by white arrow) were identified by the following criteria: Hoechst+, pan-CK+ and CD45-. Scale bar: 20 µm (B) Box plot summary indicating the range of CK+cells/ml recovered from the sample outlet for blood samples extracted from healthy volunteers, as wells as breast and lung cancer patients. The box plot presents the median, lower and upper quartiles (25th,75th percentiles). Data points that lie outside the 10th and 90th percentiles are shown as outliers (Anova, p<0.001). Encapsulated image of PAP stained isolated cells shows a large CTC with high nucleus to cytoplasmic (N/C) ratio (labeled with white arrow). (C) Staining of CTC for pan-CK and CD45. Upper panel depicts a representative image of cells which were double positive (CK+/CD45+); while lower panel shows a double negative (CK-/CD45-) cell. Scale bar: 20 µm (D) Staining of CTC for pan-cytokeratin and EpCAM. Scale bar: 20 µm.
Figure 3.
Downstream analysis of enriched CTCs.
(A) CTC viability demonstrated by attachment to 2D Geltrex (Invitrogen)-coated substrate (72 hr after seeding). Isolated CTC were enriched for CD44. No cells were stained for CD45, indicating the absence of WBCs which did not adhere to substrate and were removed after washing with 1X PBS. Some CD44+cells were not stained for Hoechst (white arrows). Scale bar: 20 µm (B) Comparison of CTC isolation and recovery with CellSearch system. (C) Molecular FISH analysis on enriched CTCs of a patient with NSCLC. Cells were stained using Vysis ALK Break Apart FISH probe and counterstained with DAPI. The red and green signals demonstrated a distinct separation of the original fusion signal (arrows), indicating a rearrangement in the 2p23 ALK-gene locus. Scale bar: 16 µm. (D) MassArray spectra for a patient with NSCLC harboring EGFR L747_P753>S. Trace from FFPE, plasma and pooled CTCs illustrated. Percentage indicates calculated proportion of mutant allele against wild type allele (UEP: Unextended primer). (i) iPlex bi-allelic spectra on FFPE sample (33% mutant frequency), (ii) iPlex bi-allelic spectrum on plasma sample (32% mutant frequency), (iii) SABER mutant specific spectrum on plasma sample (Positive – high frequency), (iv) SABER mutant specific spectrum on CTCs (Positive – low frequency (n = 3/94), estimated mutant frequency of 1.4%) and (v) Representative iPlex & SABER (shown) spectrum on no-template control sample (Negative).