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Figure 1.

Known distributions (shown in red) and sample locations (shown in black) for Octopus tetricus, (east Australia), O. cf. tetricus (Western Australia) and O. gibbsi (New Zealand).

Location acronyms: WP = Woodman's Point, MA = Mandurah, AL = Albany, ES = Esperance, CG = Cape Le Grand, FI = Flinders Island, Tasmania, WL = Wallaga Lake, NA = Narooma, PS = Port Stephens, LE = Leigh, New Zealand.

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Table 1.

Description of morphological measurements recorded.

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Figure 2.

Bayesian topology depicting the phylogenetic relationships among five currently accepted species of Octopoda.

Analyses are based on five combined partial mitochondrial genes (12s rRNA, 16s rRNA, COI, COIII and Cytb) showing bootstrap values ≥ 50 below the node and posterior probability values ≥ 0.7 above the node. Outgroup is comprised of Octopus oculifer and O. mimus. Node labels reflect locations represented by individuals contributing to node (Western Australia, 1 = Mandurah, 2 = Woodman's Point, 3 = Albany, 4 = Cape Le Grand, 5 = Esperance; East Australia, 1 = Wallaga Lake, 2 = Port Stephens, 3 = Narooma; South Africa, 1 = Port Elizabeth, 2 = Umhlanga, 3 = Hout Bay, 4 = Durban).

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Figure 3.

Generalised Mixed Yule Coalescent (GMYC) Bayesian topology depicting the phylogenetic relationships of Octopus tetricus (east Australia and Tasmania), O. cf. tetricus (Western Australia) and O. gibbsi (New Zealand).

Analysis is based on three concatenated partial mitochondrial genes (COI, COIII and Cytb). Three species clades were supported via GMYC analysis; East Australia and New Zealand (red) and Western Australia (purple and black). Node labels reflect locations represented by individuals contributing to node (Western Australia, 1 = Mandurah, 2 = Woodman's Point, 3 = Albany, 4 = Cape Le Grand, 5 = Esperance; East Australia, 1 = Wallaga Lake, 2 = Port Stephens, 3 = Narooma).

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Figure 4.

Principal component biplot of male individuals of the tetricus complex.

X axis represents PC1 (explaining 73.6% of total variation) and is driven primarily by the SCR3 and ALR3. Y axis represents PC2 (explaining 13.7% of total variation) and is driven primarily by WD and HW.

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Table 2.

Summary of mature male morphological measurements taken from preserved museum specimens of Octopus tetricus (east Australia and Tasmania), O. cf. tetricus (Western Australia and O. gibbsi (New Zealand).

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Table 3.

Male Discriminant Function Analysis: Jackknifed classification matrix.

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Figure 5.

Principal component biplot of female individuals of the tetricus complex.

X axis represents PC1 (explaining 70.7% of total variation) and is driven primarily by HW and SCL3. Y axis represents PC2 (explaining 29.3% of total variation) and is driven primarily by SCR3 and ALL3.

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Table 4.

Summary of mature female morphological measurements taken from preserved museum specimens of Octopus tetricus (east Australia and Tasmania), O. cf. tetricus (Western Australia) and O. gibbsi (New Zealand).

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Table 4 Expand

Table 5.

Female Discriminant Function Analysis: Jackknifed classification matrix.

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Table 5 Expand