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Figure 1.

Drosophila melanogaster raised on food containing Truvia show decreased longevity.

Truvia is red, Purevia is green, control nutritive sugars are dark blue, and other non-nutritive sugars are light blue. Graph shows percentage of living adult flies raised on food containing different nutritive sugars and non-nutritive sweeteners over time. Note significant decrease in longevity of adult flies raised on food containing Truvia compared to other food.

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Figure 2.

Drosophila melanogaster raised on food containing Truvia show decreased motor behavior.

Truvia is red, Purevia is green, control nutritive sugars are dark blue, and other non-nutritive sugars are light blue. Graph shows climbing ability of adult flies raised on food containing different nutritive sugars and non-nutritive sweeteners over time. Note the significant decrease in climbing behavior of adult flies raised on food containing Truvia compared to other food.

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Figure 3.

Drosophila melanogaster raised on food containing erythritol show decreased longevity.

Truvia is orange, erythritol is red, Purevia is green, and the control nutritive sugar sucrose is dark blue. Graph shows percentage of living adult flies raised on food containing sucrose and non-nutritive sweeteners over time. Note significant decrease in longevity of adult flies raised on food containing either Truvia or erythritol compared to either Purevia or sucrose.

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Table 1.

Tests for sex differences in survival of flies consuming erythritol.

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Table 1 Expand

Figure 4.

Increasing concentrations of erythritol show decreased longevity in Drosophila melanogaster.

Graph shows percentage of living adult flies raised on food containing different concentrations of erythritol. Control food is 0.5(blue line). 2 M erythritol (red line), 1 M erythritol (orange line), 0.5 M erythritol (green line), and 0.1 M erythritol (black line) were used. Note significant decrease in longevity of adult flies as concentration of erythritol is increased.

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Figure 5.

Drosophila melanogaster consume erythritol when given access to sucrose in a two-way choice experiment.

Graph shows percentage of living adult flies when given a choice between two different food sources throughout their lifespan. Negative control choice experiments provide 1(blue line). Positive control choice experiments provide 1 M erythritol on both sides of the choice chamber (orange line). Experimental groups provide 1 M erythritol on one side of the choice chamber and 1 M sucrose on the opposite side of the chamber (green line); and 2 M erythritol on one side of the choice chamber and 1 M sucrose on the opposite side of the chamber (red line). Note significant decrease in longevity in both experiments where erythritol is provided as as a choice with sucrose.

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Figure 6.

CAFE experiments show Drosophila melanogaster actively consume erythritol over time.

Upper graph shows prandial behavior of 10 individually housed flies fed 5% erythritol (red colums) and 10 individually housed flies fed 5% sucrose (blue columns) over a 6 hour period. Average intake per fly per hour is graphed for each treatment and is separated by sex. Lower graph shows prandial behavior of 10 indivually housed flies when presented with a choice between 5% erythritol (red colums) and 5% sucrose (blue columns). Average intake per fly per hour is graphed for each treatment and is separated by sex. Note the significant increase in erythritol intake compared to sucrose intake for both sexes.

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