Figure 1.
Taxonomic characterization of the producing microbe.
(A) Phase contrast microphotograph of the strain Alteromonas macleodii PA2. (B) Neighbour-joining tree based on 16S rRNA gene sequences showing the phylogenetic relationship.
Table 1.
Details of the isolates screened for production of exopolysaccharide.
Figure 2.
Fermentative production of exopolysaccharide.
(A) Effect of different carbon sources on exopolysaccharide elaboration and biomass production by Alteromonas macleodii PA2. (B) Time course of exopolysaccharide production at different concentrations of lactose by Alteromonas macleodii PA2.
Table 2.
EPS elaboration and biomass production at variable concentrations of lactose at 72Alteromonas macleodii PA2.
Figure 3.
Biophysical characterization of the silver nanoparticle synthesized using the purified polysaccharide.
(A) Uv-Vis spectra of sliver nanoparticles after incubation of the EPS in 9 mM solution of silver nitrate. (B) TEM image recorded from drop-coated film of AgNPs suspension after 6 hours of incubation. Scale bar corresponds to 50 nm.
Figure 4.
Histogram indicating size distribution by number (A) and intensity (B) of nanoparticles formed after 6 hrs of incubation.
Figure 5.
FT-IR analysis to decipher mechanism of nanoparticle biosynthesis.
(A) FT-IR spectra of the polysaccharide produced by A.macleodii PA2 (B) FT-IR spectra of the AgNP produced and stabilized by the polysaccharide.