Figure 1.
Dose effect of salicylic acid (SA) on the expression of YSL1 and YSL3 in shoots and roots of Arabidopsis thaliana ecotype Col-0.
Twelve-day-old plants were treated with SA (0, 25, 50, 100 and 200 µM) for 24 h. Quantitative real-time RT-PCR (qPCR) analysis of expression of YSL1 and YSL3 in shoots and roots relative to that of ACT2. Data are mean±SD from 9 samples from 3 biological replicates. Different letters indicate significant difference at p<0.05.
Figure 2.
Effect of exogenous SA on the expression of YSL1 and YSL3 in A. thaliana Col-0 and the npr1 mutant.
Twelve-day-old plants of A. thaliana Col-0 wild type (WT) and npr1 mutant were treated with 200 µM SA for 24 h. qPCR analysis of expression of YSL1 and YSL3 in shoots relative to that of ACT2. Data are mean±SD 9 samples from 3 biological replicates. Different letters indicate significant difference at p<0.05.
Figure 3.
Mutation in YSL3 results in enhanced susceptibility to P. syringae pv. tomato (Pst) DC3000 infection.
A, Disease symptoms on leaves of each Arabidopsis line after inoculation with Pst DC3000. Four-week-old Arabidopsis thaliana Col-0 wild type (WT), ysl1, ysl3, ysl1ysl3 and npr1 grown in the soil were spray-inoculated with 107 colony-forming units (cfu)/mL Pst DC3000 in 10 mM MgCl2 with 0.02% Silwet L-77 (hereafter Pst DC3000 solution). Photographs were taken 3 days post inoculation (dpi). B, Bacterial population in leaves of each Arabidopsis line 0, 1, and 3 days after inoculation. Data are mean±SD from 6 replicates. Different letters indicate significant difference at p<0.05.
Figure 4.
Expression of YSL1, YSL3, PR1 and SID2 in Arabidopsis leaves infected with Pst DC3000.
Four-week-old plants of Arabidopsis wild type (Col-0) were spray-inoculated with Pst DC3000 solution. Plants sprayed with 10 mM MgCl2 with 0.02% Silwet L-77 were used as a mock treatment control. Mature leaves (3rd) were collected 0, 1, 2 and 3 days post-inoculation (dpi). qPCR analysis of expression of YSL1, YSL3, PR1, SID2 and PLANT DEFENSIN1.2 (PDF1.2) relative to that of ACT2. Data are mean±SD from 6 samples of 2 biological repeats. *P<0.05 compared with mock inoculation.
Figure 5.
Effect of CORONATINE INSENSITIVE1 mutation (coi1) on the expression of YSL1, YSL3, SID2, PR1 and PDF1.2 in Arabidopsis leaves inoculated with Pst DC3000.
Four-week-old A. thaliana ecotype Col-6 wild type (WT) and coi1-16 mutant were spray-inoculated with Pst DC3000 solution. Plants sprayed with 10 mM MgCl2 with 0.02% Silwet L-77 were used as a mock treatment control. Mature leaves (3th) were harvested at 3 dpi. qPCR analysis of expression of YSL1, YSL3, PR1, SID2 and PDF1.2 relative to that of ACT2. Data are mean±SD from 6 samples of 2 biological repeats. *P<0.05 compared with wild type.