Figure 1.
A) An example of a sectioned frozen ocular globe; B) schematic representation of the four VH withdrawal areas: A, B, C, and D.
Figure 2.
Representative 1H-NMR spectrum of a VH sample with main resonance assignments.
Figure 3.
PCA score plots of 1H-NMR VH sample spectral data.
A) PC1 vs. PC2; B) PC2 vs. PC3. The explained variance is reported in brackets. Numbers represent eye samples, letters represent topographic areas, and W stands for the entire VH (A green circles; B blue squares; C red up-pointing triangles; D yellow down-pointing triangles, W light blue diamonds). Ellipse indicates the 95% Hotelling T2 confidence region. Samples with the same number and letter are duplicates.
Figure 4.
HCA of sample distribution in the PC2 vs. PC3 score space. A tree sorted by Ward clustering.
The vertical axis reports sample distances.
Figure 5.
Plot of PLS-DA variable regression coefficients in the three topographical areas: A, B, C–D.
Table 1.
Variables with VIP >1 and the corresponding regression coefficients for each VH area as calculated by PLS-DA.