Table 1.
Primers sequences.
Figure 1.
Expression of GPR41 and GPR43 mRNA in different porcine tissues at 160 day-old.
The reverse transcription in the absence of reverse transcriptase (RTse –) was used to control for amplification from incompletely digested genome DNA. The GAPDH mRNA served as a loading control. The sizes of GPR41, GPR43 and GAPDH PCR products were 398 bp, 249 bp and 220 bp, respectively. The bands of the marker were 50 bp, 100 bp, 150 bp, 200 bp, 300 bp, 400 bp, and 500 bp from bottom to above. M: marker; L: liver; S: spleen; I: ileum; C: colon; H: heart; K: kidney; AT: adipose tissue; SM: skeletal muscle.
Figure 2.
The expression level of GPR41 and GPR43 in different tissues (liver, spleen, ileum, colon and adipose tissue, n = 18) of pig.
The vertical axis of the figure was presented as the target genes copies (GPR41 and GPR43) per 1 microgram total RNA. Data was presented as mean ± SEM. Comparisons were made between different tissues (* P<0.05; ** P<0.01).
Figure 3.
The expression level of GPR41 (left) and GPR43 (right) in liver, spleen, ileum, colon and adipose tissue at different development stages (0, 25, 35, 70, 115, 160 day) of pig (n = 3).
The vertical axis of the figure was presented as the target genes copies (GPR41 and GPR43) per 1 microgram total RNA. Data was presented as mean ± SEM. Comparisons were made between different developmental stages in each tissue (* P<0.05; ** P<0.01).
Figure 4.
Analysis of GPR41 and GPR43 in porcine different tissues by western blot using GPR41 and 43 antibodies.
β-actin was used as the loading control. M: marker; L: liver; S: spleen; I: ileum; C: colon; H: heart; K: kidney; AT: adipose tissue; SM: skeletal muscle.
Figure 5.
Immunolocalization of GPR41 and GPR43 in spleen, ileum, colon and adipose tissue.
Sections were stained with the polyclonal human-specific GPR41 and GPR43 antibody (Santa Cruz, CA). The images were captured on each slide at 400× magnification under Olympus BX51 (Olympus, Japan). The cells marked by the arrows were GPR41 or GPR43 immunoactive. Bar = 20 µm.