Figure 1.
Cotton fiber morphology in the wild-type line and the mutant lines.
a: wild line: TM-1; b: recessive naked-seed mutant: n2NSM; c: dominant naked-seed mutant: N1NSM; d: fuzzless-lintless mutants: SL1-7-1FLM (with N1 gene), XZ142FLM (with n2 gene), MD17FLM (with N1 and n2). Matured seed were separated from the opened bolls on the cotton plant. Ginned seeds (right) and matured seeds (left) showed on linted-fuzzy and linted-fuzzless panel.
Figure 2.
Dynamic progression of common differentially expressed genes in the dominant mutants.
(a) Unsupervised hierarchical clustering of the 4,358 common DEGs in the dominant mutants. Common DEGs were clustered into six groups and the number of genes of each group was listed at right. Red region, genes upregulated in the mutants; green region, genes downregulated in the mutants. A, TM-1; B, SL1-7-1FLM; D, MD17FLM; E, N1NSM; 1, +1 DPA; 2, +3 DPA; 3, +5 DPA. (b) Functional distribution of common DEGs in the dominant mutants. (c) Functional category enrichment of common differentially expressed genes in the dominant mutants.
Figure 3.
Dynamics of transcription factor expression profiles in various dominant mutants.
(a) Unsupervised hierarchical clustering of 355 transcription factor genes included in the 4,358 common DEGs in the dominant mutants. Four groups were generated and the number of each group was in parentheses. Red region, genes upregulated in the mutants; green region, genes downregulated in the mutants. A, TM-1; B, SL1-7-1FLM; D, MD17FLM; E, N1NSM; 1, +1 DPA; 2, +3 DPA; 3, +5 DPA. (b) Representative functions and genes showing expression gradients. (c) Distribution of transcription factor families among G1, G2, G3 and G4.
Figure 4.
Dynamic progression of common differentially expressed genes in the recessive mutants.
(a) Unsupervised hierarchical clustering of 6,693 common differentially expressed genes in the recessive mutants. Common DEGs were clustered into six groups and the number of genes of each group was listed at right. Red region, genes upregulated in the mutants; green region, genes downregulated in the mutants. A, TM-1; C, XZ142FLM; F, n2NSM; 1, +1 DPA; 2, +3 DPA; 3, +5 DPA. (b) Functional category distribution of common DEGs in the recessive mutants. (c) Functional category enrichment of common differentially expressed genes in the recessive mutants.
Figure 5.
Dynamics of transcription factor accumulation profiles in various recessive mutants.
(a) Unsupervised hierarchical clustering of 506 transcription factor genes included in the 6,693 common DEGs in the recessive mutants. Four groups were generated and the number of each group was in parentheses. Red region, genes upregulated in the mutants; green region, genes downregulated in the mutants. A, TM-1; C, XZ142FLM; F, n2NSM; 1, +1 DPA; 2, +3 DPA; 3, +5 DPA. (b) Representative functions and genes showing expression gradients. (c) Distribution of transcription factor families among G1, G2, G3 and G4.
Figure 6.
Dynamic progression of common differentially expressed genes in the dominant/recessive mutants.
(a) Hierarchical clustering of the 1,932 common DEGs in five mutants. Common DEGs were clustered into five groups and the number of genes of each group was listed at right. Red region, genes upregulated in the mutants; green region, genes downregulated in the mutants. A, TM-1; B, SL1-7-1FLM; C, XZ142FLM; D, MD17FLM; E, N1NSM; F, n2NSM; 1, +1 DPA; 2, +3 DPA; 3, +5 DPA. (b) Functional distribution of common DEGs in the dominant/recessive mutants. (c) Functional category enrichment of common DEGs in the dominant/recessive mutants.
Figure 7.
Dynamics of transcription factor accumulation profiles in various dominant/recessive mutants.
(a) Unsupervised hierarchical clustering of 153 transcription factor genes included in the 1,932 common DEGs in the recessive mutants. Four groups were generated and the number of each group was in parentheses. Red region, genes upregulated in the mutants; green region, genes downregulated in the mutants. A, TM-1; B, SL1-7-1FLM; C, XZ142FLM; D, MD17FLM; E, N1NSM; F, n2NSM; 1, +1 DPA; 2, +3 DPA; 3, +5 DPA. (b) Representative functions and genes showing expression gradients. (c) Distribution of transcription factor families among G1, G2, G3 and G4.
Figure 8.
QRT-PCR confirmation for the six selected differentially-expressed transcription factor genes.
The expression level of selected genes at +1 DPA, +3 DPA and +5 DPA in TM-1, N1NSM and n2NSM. Data shown are the means ± SD of three biological replicates.
Figure 9.
Model for the action of GL1-activating trichome development in Arabidopsis thaliana and fuzz development in Gossypium hirsutum.
a: Model for the action of GL1-activating trichome development in Arabidopsis thaliana. b: Model for the action of GL1-activating fuzz development in Gossypium hirsutum A: TM-1, B: SL1-7-1FLM, C: XZ142FLM, D: MD17FLM, E: N1NSM, F: n2NSM, 1: +1 DPA, 2: +3 DPA, 3: +5 DPA. Light red/green bars indicate cotton fiber gene expression in the upper/lower group.