Figure 1.
From the left, a polarizer at an angle of 45°, a Nomarski prism, a condenser, a sample, an objective lens, a Nomarski prism, and an analyzer at 135° are aligned. After passing through the first prism, the illuminated beam is split into two different polarizations. Retardation due to the thickness variation of samples generates interference after the analyzer.
Figure 2.
DIC microscopy and retinal-section images with progress of RP (rats).
The top-left image (A) shows the normal state of the photoreceptor layer, and the top-right image (B) shows a corresponding retinal section of the specially functionalized retinal layer including the GCL, IPL, INL, OPL, ONL, IS+OS, and RPE. (C) and (D) show the states aged 5 weeks, and (E) and (F) show the states aged 8 weeks. Morphological changes with RP progression were observed in both imaging modalities.
Figure 3.
DIC microscopy and retinal-section images with progress of RP (mice).
The top-left image (A) shows the normal state of the photoreceptor layer, and the top-right image (B) shows a corresponding retinal section. (C) and (D) show the states aged 3 weeks, and E and F show the states aged 3 months. Morphological changes with RP progression were observed in both imaging modalities.
Figure 4.
(A) and (B) show original DIC images of the normal and progressed RP (5 weeks) states, respectively. (C) and (D) show counted cell boundaries analyzed by MATLAB using a morphological dilation and erosion algorithm. (E) and (F) show the combined images of original and analyzed images, which indicate well-matched positions.