Figure 1.
qPCR analysis of the effect of m6A on DNA amplification.
qPCR analysis using different concentrations of the DNA templates ACTB (A), HPRT1 (B) and HSPA8 (C) containing m6A (open squares) or unmethylated adenosine (black circles) revealed that m6A does not interfere with DNA amplification.
Figure 2.
Differences in gene expression between WT and FTO-4 mice based on microarray data.
Expression of the indicated genes in the cerebellum (A), hypothalamus (B), WAT (C) and gastrocnemius (D) of wildtype (WT, black bars) and FTO-4 (white bars) mice. Data are expressed as the fold change compared to wildtype. Significance was tested using Student's t-test to compare FTO-4 to WT. ***p<0.001, **p<0.01, *p<0.05.
Table 1.
Top 10 genes that showed the highest amount of overexpression in FTO-4 mice per tissue.
Table 2.
Top 10 genes that showed the highest amount of downregulation in FTO-4 mice per tissue.
Table 3.
Obesity-associated genes that show altered expression in FTO-4 mice.
Figure 3.
Effect of FTO overexpression on m6A levels in RNA.
FTO mRNA expression in wild-type (black bar) and FTO-4 (white bar) MEFs as the fold change compared to wild-type (A). m6A levels measured as a percentage of adenosine levels for mRNA and total RNA by LC/MS in wild-type (black bar) and FTO-4 (white bar) MEFs (B). Significance was tested using Student's t-tests to compare FTO-4 to WT data. **p<0.01.
Figure 4.
Overview of potential m6A sites in obesity-related genes with changed expression in FTO-4 mice.
Pie chart of obesity-related genes whose expression is changed in the indicated tissues of FTO-4 mice. Dark grey, m6A sites in their mRNA transcripts [23], [24]. Light grey, no m6A sites [23], [24]. The number of genes involved is indicated: see Table 2 for gene names.