Figure 1.
Summary of the sequential analyses performed in this study.
Figure 2.
Venn diagrams showing the number of genes that were differentially expressed.
The diagrams show the genes that are exclusive to each treatment and those that are shared by two or all three experimental groups (Cas, E2 and Cas+E2) compared to the control group.
Figure 3.
Number of enrichment terms exclusive or shared among groups.
The three treatments shared 90 enrichment terms. The highest number of enrichment terms was found in the Cas group. The Cas+E2 group showed no exclusive enrichment term.
Figure 4.
Differentially expressed TF and transcription regulation-related genes.
Transcription regulation-related genes indentified by the IPA Ingenuity program and showing differential expression in those subgroups containing enrichment terms. Red and green colors highlight the up- or down-regulated genes as compared with the control group, respectively. A minimum spanning tree is used to show the correlation among the expression variation of the differentially expressed genes (yellow) plus the androgen receptor (AR) and estrogen receptors (ESR1 and ESR2) (red) in human tissues. Each gene is indicated as a vertices and their size represent the standard deviation of their fold changes in different tissues. The edge length is the distance calculated from the correlation matrix as explained in M&M. Of note, are the centrality of TMF1 and the short distances between the pairs AR-NKX3-1, ESR1-BRCA2, and ESR2-NFKB2.
Table 1.
The eighty most regulated genes.
Figure 5.
TF with possible binding sites found in the 80 most-regulated genes.
(A) TF binding sites not found in the internal control genes. (B) TF binding sites also found in the proximal promoter of internal control genes. Density values≤0.01 were omitted.
Figure 6.
Kinetics of Mybl1, Mybl2 and Gata2 expression.
Quantitative RT-PCR determination of the mRNA content for the Mybl1 (A), Mybl2 (B) and Gata2 (C) genes in the prostate of non-castrated (NC) and castrated rats up to 7 days after surgery. The fold-change variation with respect to the controls is shown as the mean ± the standard variation (n = 3 for each time point). The asterisks indicate p<0.05. The dotted lines in each figure correspond to the fourth-power exponential fitting curve, and are shown together for the sake of direct comparison in D.
Figure 7.
Kinetics of Nfkb1, Nfkb2, Rel and RelA expression.
Quantitative RT-PCR determination of the mRNA content for the Nfkb1 (A), Nfkb2 (B), Rel (c-Rel) (C) and RelA (D) genes in the prostate of non-castrated (NC) and castrated rats up to 7 days after surgery. The fold-change variation with respect to the controls is shown as the mean ± standard variation (n = 3 for each time point). The asterisks indicate p<0.05. The dotted lines in each figure correspond to the fourth-power exponential fitting curve, and are shown together for the sake of direct comparison in E.
Figure 8.
Kinetics of Evi1, Elk1 and Nfyb expression.
Quantitative RT-PCR determination of the mRNA content for the Evi1 (A), Elk1 (B) and Nfyb (C) genes in the prostate of non-castrated (NC) and castrated rats up to 7 days after surgery. The fold-change variation with respect to the controls is shown as the mean ± standard variation (n = 3 for each time point). The asterisks indicate p<0.05. The dotted lines in each figure correspond to the fourth-power exponential fitting curve, and are shown together for the sake of direct comparison in D.
Figure 9.
TF localization in epithelial and stroma cells.
Immunohistochemical localization of selected TF (red) as indicated in the prostate of non-castrated controls (NC) and castrated rats 3 days after surgery (CAS). MYBL2 (A,B), GATA2 (C,D), EVI1 (E,F), ELK1 (G,H), NFYB (I,J), NFKB1 (K,L), NFKB2 (M,N), REL (O,P), RELA (Q,R), RELB (S,T). Nuclei were stained with DAPI (blue). White arrows indicate smooth-muscle cells. Yellow arrowheads indicate cells showing nuclear location of the TF. L = gland lumen; S = stroma. Scale bars = 50 µm.