Figure 1.
Citric acid-induced Ca+2 influx in (A) CCD19-Lu cells, (B) A549 cells and (C) hTRPA1/CHO cell line.
Each point in the graph is an average ± SEM of at least two independent experiments.
Figure 2.
Blockade of citric acid-induced Ca+2 influx by TRPA1 and TRPV1 antagonists.
(A–B) Inhibition of citric acid-induced Ca+2 influx by GRC 17536, compound 7 and GRC 6211 in CCD19-Lu (A) and A549 (B) cells. (C) Inhibition of citric -induced Ca+2 influx by GRC 17536, compound 7 and GRC 17770 in hTRPA1/CHO cells. Each point in the graph is an average ± SEM of at least two independent experiments.
Table 1.
In vitro TRP selectivity profile of GRC 17536 and compound 7.
Figure 3.
Effect of TLR4, TLR7 and TLR8 ligand on TRPA1 activation.
(A) Effect of LPS on TRPA1 agonist (crotonaldehyde, H2O2 and 15d-PGJ2)-mediated 45Ca+2 uptake in hTRPA1/CHO cells. (B) Effect of loxoribine and ssRNA40 on AITC mediated 45Ca+2 uptake in hTRPA1/CHO cells. (C) Effect of LPS, loxoribine and ssRNA40 on AITC mediated 45Ca+2 uptake in A549 cells. (D) LPS, loxoribine and ssRNA40 enhance TRPA1 expression. RT-PCR was performed with mRNA isolated from hTRPA1/CHO cells. Each histogram is the mean ± SE of at least two independent experiments.
Figure 4.
Blockade of TLR agonist-mediated enhanced 45Ca+2 uptake by TRPA1 antagonists in (A) hTRPA1/CHO cells and (B) A549 cells.
Each histogram is the mean ± SE of at least two independent experiments.
Figure 5.
Effect of GRC 17536 on citric acid (0.4 M) induced cough response in male guinea pigs.