Figure 1.
Images of representative plants of the 19 parental lines [1] at 28 DAS.
(A) EDi-0, (B) Can-0, (C) WS-0, (D) Hi-0, (E) Zu-0, (F) Po-0, (G) Sf-2, (H) Wil-2, (I) 0y-0, (J) Kn-0, (K) Ct-1, (L) Wu-0, (M) Tsu-0, (N) Col-0, (O) Rsch-4, (P) No-0, (Q) Bur-0, (R) Ler-0 and (S) Mt-0.
Figure 2.
Example features extracted from one plant of EDi-0.
(A) normsmallpax and lormlargepax, (B) vrectsizex and vrectsizey, (C) maxdiam, (D) outline of convex hull, from which convhullcirc and convhullarea are determined (E) minrectarea, (F) mincirclediam.
Table 1.
Shape descriptors extracted from segmented Arabidopsis rosettes.
Figure 3.
Time course for selected genotypes from statistical model for (A) loge Area (B) loge Circumference (C) Compactness and (D) Roundness.
Figure 4.
Multiple comparisons of Compactness and Area between ecotypes.
Scatter plots showing significant (P>0.05•, P<0.05 •, P<0.01•, Post-hoc Tukey test between ecotypes) differences between ecotypes for (A) ‘Compactness’ and (C) ‘Area’ and boxplots for (B) ‘Compactness’ and (D) Area over time. Images have been added to (A) and (B) to show range of ‘Compactness’ and ‘Area’.
Figure 5.
Shape descriptor plots of ecotype's feature profile.
Radar plot of 20 shape features for each ecotype. Plots are average of 12 replicates over five time points. Variable assignment key for star plot is located at the bottom right corner. Each star plot or segment diagram represents one row of the input data. Variables (columns) start on the right and wind counter clockwise around the circle. The size of the (scaled) column is shown by the distance from the centre to the point on the star or the radius of the segment representing the variable. The columns of the data matrix are scaled independently so that the maximum value in each column is 1 and the minimum is 0. Segment numbers corresponding to descriptors are defined in Table 1.
Figure 6.
(A) Variable loadings for first and second principal components, 17 DAS and (B) Analysis by ecotype over first and second principal components, 17 DAS. Significance level is given by the size of the squares, the smaller the square the more significantly different (P<0.05). (C) Contrast in ‘Area’ between ‘Bur-0’ and ‘Wu-0’ and Contrast in ‘Compactness’ between ‘Ct-1’ and ‘No-0’) and where both ecotypes have similar Area as demonstrated in plot (A).
Figure 7.
(A) Relative Rosette Area Growth Rate Over Time (RRAGR), (B) Compactness.