Table 1.
Chemical parameters of seawater carbonate system.
Figure 1.
Specific growth rates of P. tricornutum.
Growth rates of P. tricornutum cells grown under the LC (390 ppmv, pH 8.18) and HC (1000 ppmv, pH 7.82) and then both acclimated to different light levels (60, 200 and 460 µmol m−2 s−1) for 25–36 generations. Values are means ± SD, n = 3. The short-lines above the histogram bars indicate significant difference between LC and HC, and the different letters indicate significant differences among the light treatments within the HC- or LC-grown cells at p<0.05.
Figure 2.
Pigmentation of P. tricornutum.
Chl α (A), Carotenoids (B) and Chl c (C) of P. tricornutum cells grown under the LC (390 ppmv, pH 8.18) and HC (1000 ppmv, pH 7.82) and then both acclimated to different light levels (60, 200 and 460 µmol m−2 s−1) for 25–36 generations. Values are means ± SD, n = 3. The short-lines above the histogram bars indicate significant difference between LC and HC, and the different letters indicate significant differences among the light treatments within the HC- or LC-grown cells at p<0.05.
Table 2.
Ratios between carotenoids, Chl c and Chl a concentrations of P. tricornutum.
Figure 3.
The effective photochemical yield (Fv
′/Fm′) of P. tricornutum. The effective photochemical yield (Fv′/Fm′) of P. tricornutum cells grown under the LC (390 ppmv, pH 8.18) and HC (1000 ppmv, pH 7.82) and then both acclimated to different light levels (60, 200 and 460 µmol m−2 s−1) for different generations (from 0 to 36). Values are means ± SD, n = 3.
Table 3.
The fitted parameters derived from the rapid light curves of P. tricornutum.
Figure 4.
Non-photochemical quenching of P. tricornutum.
The changes in non-photochemical quenching of P. tricornutum cells grown under the LC (390 ppmv, pH 8.18) and HC (1000 ppmv, pH 7.82) and then both acclimated to different light levels (60, 200 and 460 µmol m−2 s−1) for 25–36 generations under high actinic light (1228 µmol m−2 s−1). Values are means ± SD, n = 3. The short-lines above the histogram bars indicate significant difference between LC and HC, and the different letters indicate significant differences among the light treatments within the HC- or LC-grown cells at p<0.05.
Figure 5.
Half-saturation constants (K1/2) for DIC (A) and CO2 (B) of P. tricornutum.
Half-saturation constants (K1/2) for DIC (A) and CO2 (B) of P. tricornutum cells grown under the LC (390 ppmv, pH 8.18) and HC (1000 ppmv, pH 7.82) and then both acclimated to different light levels (60, 200 and 460 µmol m−2 s−1) for 25–36 generations under middle actinic light (830 µmol m−2 s−1). Values are means ± SD, n = 3. The short-lines above the histogram bars indicate significant difference between LC and HC, and the different letters indicate significant differences among the light treatments within the HC- or LC-grown cells at p<0.05.
Figure 6.
The changes in quantum yield (Fv/Fm) of P. tricornutum.
The changes in quantum yield of P. tricornutum cells grown under the LC (390 ppmv, pH 8.18) and HC (1000 ppmv, pH 7.82) and then both acclimated to different light levels (A: 60 µmol m−2 s−1; B: 460 µmol m−2 s−1) for 25–36 generations under dark or dim light (10 µmol m−2 s−1). Values are means ± SD, n = 3.
Table 4.
Mean specific growth rates of P. tricornutum.