Figure 1.
Tissue selection pipeline for finding MTI-supported tissues of miR-17 based on experimentally validated MTIs.
Experimentally validated MTIs sharing the same miRNA were selected from the miRTarBase database. The correlations of MTIs across a combination of tissues, which were selected by our method, are strongly negative.
Table 1.
Tumor tissues and normal tissues.
Table 2.
The list of miRNAs with equal to or more than 10 targets among 743 experimentally validated MTIs and the average correlations.
Figure 2.
The flowchart of the proposed algorithm for finding MTI-supported tissues.
Figure 3.
The loss of function values for different numbers of MTI-supported tissues.
Table 3.
The MTI-supported tissues from rank 1 to 8.
Figure 4.
The correlation density plot and heatmap for experimentally validated MTIs (miRTarBase).
a. Comparison of correlation densities for all 743 experimentally validated MTIs (solid line) and hsa-miR-17 with 24 targets across the top 6 MTI-supported tissues (dashed line). b. The expression profiles of hsa-miR-17 and 24 targets across the top 6 MTI-supported tissues. The correlations between the expression profile of hsa-miR-17 and the profiles of target genes were annotated next to the gene symbol. Green represented low expression and red represented high expression in corresponding genes and tissues.
Figure 5.
Comparison of correlation densities for all 743 experimentally validated MTIs across all 89 tissues (black solid line), hsa-miR-17 with 24 targets across the top 6 MTI-supported tissues (red dashed line), hsa-miR-17 with 24 targets across 19 extended tissues (green dashed line) and hsa-miR-17 with 95 predicted targets across the top 6 MTI-supported tissues (blue dashed line).
Figure 6.
The heatmaps of hsa-miR-17 expression and regulated genes.
a. The expression profiles of hsa-miR-17 and 24 targets across 19 extended tissues. The correlations between the expression profiles of hsa-miR-17 and target genes were annotated next to the gene symbols. Green represented low expression and red represented high expression in corresponding genes and tissues. b. Part of the expression profile of hsa-miR-17 and experimentally validated and predicted target genes. There are 20 negatively correlated target genes; 16 of these target genes are predicted to be hsa-miR-17 target genes. PTEN, MAPK9, MAP3K12 and BMPR2 are experimentally confirmed target genes.
Table 4.
The list of the selected MTI-supported tissues corresponding to 19 miRNAs with equal to or more than 10 targets among 743 experimentally validated MTIs.