Table 1.
Comparative analysis of member P450s in selected P450 families across 21 basidiomycete species.
Figure 1.
Comparative analysis of amino acid patterns in P450 signature motif EXXR in fungal P450s.
A total of 4304 P450s from the fungal phyla ascomycota (1336 P450s), basidiomycota (2859 P450s), zygomycota (102 P450s) and chytridiomycota (7 P450s) were used for the analysis. A total of 128 amino acid patterns for the EXXR motif were identified. The predominant amino acid patterns (>2%) are shown in the pie charts. Amino acid patterns are shown in the figure along with the number of amino acids representing the pattern and overall percentage of that pattern. A detailed list of amino acid patterns occurring at P450 signature motifs is shown in Table S3.
Table 2.
Analysis of P450 signature motifs EXXR and CXG in fungal P450s.
Figure 2.
Comparative analysis of amino acid patterns at the P450 signature motif CXG in fungal P450s.
In total 4304 P450s from the fungal phyla ascomycota (1336 P450s), basidiomycota (2859 P450s), zygomycota (102 P450s) and chytridiomycota (seven P450s) were used for the analysis. As shown in the figures, 32 amino acid patterns were identified in fungal P450 for the CXG motif. The numbers on the bars represent the number of amino acids representing the pattern. The overall percentage of that pattern is shown in parenthesis.
Figure 3.
Analysis of amino acid patterns at EXXR motif in 12 fungal P450 families.
A sequence logo for the EXXR motif using the amino acids from 12 fungal P450 families was constructed, as described in the “Materials and methods” section. The number of P450s used for the construction of the sequence logo is shown in the parenthesis right next to the name of the P450 family.
Figure 4.
Analysis of amino acid patterns at CXG motif in 12 fungal P450 families.
Sequence logo for the CXG motif (FXXGXRXCXG) using the amino acids from 12 fungal P450 families were constructed as described in the “Materials and methods” section. The number of P450s used for construction of the sequence logo is shown in the parenthesis right next to the name of the P450 family.
Table 3.
Comparative quantitative analysis of amino acid patterns at EXXR and CXG (FXXGXRXCXG) motifs in 12 fungal P450 families.
Table 4.
Comparative quantitative analysis of amino acid patterns at EXXR and CXG (FXXGXRXCXG) motifs in 54 P450 families.
Figure 5.
Analysis of amino acid patterns at the EXXR and CXG motif in CYP51 P450 family.
In total 407 CYP51 P450 sequences representing all biological kingdoms were analyzed for EXXR and CXG signature sequences. Member P450 sequences were retrieved from the CYPED website (26) and used for analysis, as described in the “Materials and methods” section.