Table 1.
Descriptions of candidate reference genes, their primer sequences, product sizes and amplicon characteristics.
Figure 1.
Range of Ct values of the candidate reference genes obtained from all samples.
Each box corresponding to GAPDH, EF1α, ACT2, β-TUB, UBQ9, GRP, GLU1, CYP, EIF4A and UBQ7 indicates the 25% and 75% percentiles. Whiskers represent the maximum and minimum values. The median is depicted by the line across the box.
Figure 2.
Gene expression stability and ranking of potential reference genes as calculated by geNorm.
Samples are treated or directly harvested from the experimental conditions: (A) total, (B) abiotic stresses, (C) cold, (D) heat, (E)PEG, (F)salinity, (G) hormones, (H) different tissues.
Figure 3.
Determination of the optimal number of reference genes for normalization by pairwise variation using geNorm.
Table 2.
Ranking of candidate reference genes according to their expression stability value calculated by NormFinder.
Table 3.
Ranking of candidate reference genes in order of their expression stability as calculated by BestKeeper.
Figure 4.
Relative quantification of WRKY50 expression using the selected reference gene(s).
Relate expression of WRKY50 was normalized using the single most stable reference gene EF1α, β-TUB and their combination EF1α +β-TUB in sample sets across (A) abiotic stresses, (B) hormone application, and (C) different tissue types.