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Figure 1.

Longitudinal MR images of a lung tumor in an individual mouse.

No lesion was detected at 12 weeks after NNK treatment (a). At 18 weeks, a small lesion was identified (b). This lung tumor increased in size over time, at 24 (c) and 30 weeks (d). The lower panels (e, f, g, h) are the corresponding axial reformatted images for a, b, c, and d, respectively. The lesion was found to be an adenoma by histological examination.

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Figure 1 Expand

Figure 2.

The MRI size of each lesion at each time point.

Three lesions were detected at 18 weeks and grew in size over the following time. A total of 11 lesions was detected at 24 weeks and kept growing in size until 30 weeks, the end of the study.

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Figure 2 Expand

Figure 3.

Representative coronal T1-weighted MR images of lung tumors.

Two lung tumors located in the right lung (small arrowhead and arrow) and one lung tumor located in the left lung (large arrowhead) (a). Three-dimensional volume-rendered image generated from the 3D data showing the relative locations of tumors and pulmonary vessels (b).

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Figure 3 Expand

Figure 4.

Hematoxylin and eosin staining of lung lesions.

Panels a, b, and c show histological images of the lung lesions in Figure 2, indicated by the small arrowhead, long arrow, and large arrowhead, respectively. The lesions were found to be adenomas (a and c) and a carcinoma (b) by histological examination.

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Figure 4 Expand

Figure 5.

Correlation of tumor size determined by MR imaging and histological evaluation (correlation coefficient = 0.87).

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Figure 5 Expand