Table 1.
Production, or not, of sclerotia by Aspergillus niger strain IBT 29019, IBT 24631, and IBT 24634, and production of apolar indoloterpenes on Czapek yeast autolysate agar with or without added plant parts.
Table 2.
Aspergillus section Nigri: Production of sclerotia on CYA agar or CYA with raisins (most sclerotia were formed around the raisins on the agar surface, and few sclerotia if any on the raisins themselves, see footnotes).
Figure 1.
Numerous cream-coloured sclerotia produced on CYAR agar can be seen surrounding the raisins and the usual heavy sporulation caused by black Aspergillus niger IBT 29019 heads.
The ellipses added show the position of the raisins, which are covered with black aspergilla.
Figure 2.
Approximately 41 white sclerotia can be observed on a close-up of Aspergillus niger IBT 29019 growing on Czapek yeast autolysate agar with raisins.
Note the clear or brown exudate droplets associated with most sclerotia.
Figure 3.
Ultra high performance liquid chromatography time of flight high resolution mass spectrometry electrospray ionization + base peak (UHPLC-TOF-HRMS ESI+ BP) chromatogram of Aspergillus niger (IBT 29019) extracts.
A: Plug extraction from growing and sporulating culture with no sclerotium production (CYA agar with biotin). B: Plug extraction from growth with sclerotium production (CYAR with biotin). C: Sclerotium extraction (from CYAR with biotin). 1) Nigragillin, 2) Pyranonigrin A, 3) Fonsecin, 4) Aurasperone/nigerasperone analog, 7) Tensidol B, 11) Kotanin, 12) Flavasperone analog, 13) Rubrofusarin B, 14) Aflavinine analog, 15) Aflavinine analog, 17) Anominine, 18) 10,23-dihydro-24,25-dehydroaflavinine.
Figure 4.
Ultra high performance liquid chromatography time of flight high resolution mass spectrometry electrospray ionization + base peak (UHPLC-TOF-HRMS ESI+ BP) chromatogram of Aspergillus tubingensis (IBT 16833 = CBS 161.79 = NRRL 4700) extracts.
A: Plug extraction from colony area with no sclerotia (CYAR with biotin). B: Plug extraction from colony area with sclerotia (CYAR with biotin). C: Sclerotium extraction (from CYAR with biotin). 1) Nigragillin, 3) Fonsecin, 5) TMC-256A1, 6) Asperazine, 7) Tensidol B, 8) Fonsecin B, 9) Aurasperone C, 10) Aurasperone B, 12) Flavasperone analog, 14) Aflavinine analog, 15) Aflavinine analog, 16) Aflavinine analog, 17) Anominine, 18) 10,23-dihydro-24,25-dehydroaflavinine, 19) Aflavinine analog, 20) Aflavinine analog. From this strain TePaske et al. [20], [21], [22] isolated 14-epi-14-hydroxy-10,23-dihydro-24,25-dehydroaflavinine, 10,23-dihydro-24,25-dehydro-21-oxo-aflavinine, 10,23-dihydro-24,25-dehydroaflavinine, tubingensin A and B, and dehydrotubingensin A and B.