Figure 1.
Flow cytometric analysis of ILC3s using RORc-eYFP fatemap mice.
(a) Gating strategy for ILC3s in RORc-eYFP mice: after exclusion of doublets, live CD45+ B220− CD3− TCRβ− YFP+ cells were selected. (b) The population gated in panel a contains a significant fraction of CD5+ cells both in spleen, lung and colon. (c) In RORc-eYFP-RAG−/− mice all YFP+ CD45+ live cells stain negative for CD3, TCRβ and also CD5. Representative plots for n = 3 (mean +/− s.e.m.).
Figure 2.
CD5+ positive cells within the ILC3 gate express high levels of TRAC.
Normalized fold expression for TRAC and TRDC comparing CD5+ and CD5− cells within the ILC3 gate (“CD5+ ILC3s”, pregated on CD45+ B220− CD11b− CD3− TCRβ− YFP+ cells), αβ and γδ T cells, monocytes (Mono) purified from RORc-eYFP mice and ILC3s derived from RORc-eYFP RAG1−/− mice (ILC3s RAG1−/−). Results are presented relative to those obtained from ILC3s of RORc-eYFP RAG1−/− mice (mean +/− s.e.m.).