Table 1.
Primers for amplification of 18S rDNA from Phaeodactylum tricornutum CCMM 2004.
Figure 1.
Proportions of the four morphotypes during long-term culture.
Data are averages of duplicate measurements. Error bars represent standard deviation.
Figure 2.
Transformation from cruciform, triradiate and fusiform to oval morphotype.
This transformation involves four stages. In stage 1, the three morphotypes show the characteristics of a thin form indicating that the content of intracellular substances is poor. In stage2, the cells are plump indication many intracellular substances. In stage 3, the overall outline of the cells begins to change. In stage 4, the arms of the three morphotypes gradually disappear and the oval form is observed. Scale bars = 10 μm.
Figure 3.
Transformations between the four morphotypes.
Transformations 1–6 are study results of previous researchers. Pathway 7 observed in this study is a new discovery.
Figure 4.
Intermediate forms in the culture.
A–E are DIC photos. Scale bars = 10 μm. F is SEM result and the white arrows direct to the “conjunct arm”.
Figure 5.
Proportion of the three morphotypes at different temperatures.
Data are averages of duplicate measurements. Error bars represent standard deviation.
Figure 6.
Biomass of Phaeodactylum tricornutum cultured at different temperatures.
Data are averages of duplicate measurements. Error bars represent standard deviation.
Figure 7.
Images of Phaeodactylum tricornutum cells stained with BODIPY 505/515.
a1, a2, a3, a4 and a5 are images at white light, while b1, b2, b3, b4 and b5 are corresponding images at the exciting light. Scale bars = 10 μm.
Table 2.
Histogram statistical data for changes in fluorescence value.
Table 3.
Fatty acid composition of Phaeodactylum tricornutum cultured at different temperatures.
Table 4.
Biodiesel properties at the four culture temperatures.