Figure 1.
Nasal polyp tissue (n = 9) was pre-incubated with FF or MF at different concentrations for 1 hour and consequently stimulated with SEB for 24 hours (pre-challenge incubation).
% inhibition of cytokine release was calculated at a fixed concentration of 10−10 M; FF had a significantly higher suppressive effect on IFN-γ, IL-2 and IL-17 release vs. MF.
Table 1.
Nasal polyp tissue (n = 9) was incubated with FF or MF at different concentrations for 1 hour and subsequently stimulated with SEB for 24 hours (pre-challenge incubation).
Figure 2.
Nasal polyp tissue (n = 9) was stimulated with SEB for 1 hour before incubation with FF and MP at 10−11 to 10−8 molar concentrations for further 24 hours (post-challenge incubation).
The figures show a concentration-dependent inhibition of cytokine release, reaching significance at different concentrations per drug and cytokine. Significance is indicated vs. baseline (* p<0.05). Please refer to table 2 for a comparison between drugs.
Table 2.
Nasal polyp tissue (n = 9) was stimulated with SEB for 1 hour before incubation with FF and MP at different concentrations for further 24 hours (post-challenge incubation).
Figure 3.
Nasal polyp tissue (n = 9) was incubated with FF and MF at different concentrations for 1 hour, then washed and SEB was added either immediately (0 hour), after 6 or 24 hours (pre-challenge incubation with time interval) Cells were then incubated for another 24 hours.
When SEB stimulation was performed immediately, FF demonstrated a faster onset of action with a significantly higher maximal inhibition of all cytokines vs. MF. For most cytokines and time points, FF reaches a significant reduction at a lower concentration; however, when the time interval between pre-incubation and challenge was minimally 6 hours, the suppression capacity of MF reached the same levels as FF for IL-5 and TNF-α.
Table 3.
Nasal polyp tissue (n = 9) was incubated with FF and MF at different concentrations for 1 hour, and then washed and SEB was added immediately (0 hour), after 6 or 24 hours (pre-challenge incubation with time interval).