Figure 1.
Chemical structures of 6′-hydroxy justicidin B (HJB), 6′-hydroxy justicidin A (HJA), justicidin B (JB), chinensinaphthol methyl ether (CME), Taiwanin E methyl ether (TEME), etoposide (ETO), paclitaxel (TAX) and podophyllotoxin (POD).
Figure 2.
Effect of HJB, HJA, JB, CME, TEME, POD and ETO on K562 cell proliferation.
Cells were exposed to the indicated concentrations of arylnaphthalene lignans and incubated for 48± SD of three independent experiments, where each sample was tested in at least triplicate.
Figure 3.
Effect of arylnaphthalene lignans on SOD activity in K562 cells.
K562 cells were treated with of the indicated concentrations of HJA, HJB, JB, CME, TEME and POD for 48± SD of five independent experiments; **P<0.01 compared with control group.
Figure 4.
Effect of arylnaphthalene lignans on apoptosis in K562 cells.
(A) The apoptosis rate and changes in the cell cycle of K562 cells treated with arylnaphthalene lignans at the indicated doses for 24 h were analyzed by PI staining and subsequent flow cytometry. Representative data are shown. (B) Apoptosis was further analyzed by annexin V/PI staining and flow cytometry of K562 cells treated with the indicated concentrations of HJA, HJB, JB, CME and ETO for 48 h. Representative FACS scatter-grams are shown. All results are representative of three independent experiments.
Table 1.
The apoptosis rate and cell cycle analysis of K562 cells treated with arylnaphthalene lignans for 24
Figure 5.
Effects of arylnaphthalene lignans on caspase-3 activity in K562 cells.
Active caspase-3 expression was detected by flow cytometry in K562 cells after treatment with the indicated concentrations of HJA, HJB, JB, CME, TEME, ETO and TAX for 48 h. The results are representative of three independent experiments.