Table 1.
Origins, chromosome numbers and accession details of the Brachypodium species studied.
Table 2.
Specification of BAC clones used for comparative chromosome barcoding.
Figure 1.
Comparative BAC-FISH mapping of clones from the chromosome Bd2 to various species of Brachypodium.
Only one chromosome of a homologous pair is shown in each cell. The colour of the text label in the first column indicates the fluorochrome used (red – tetramethylrhodamine, green – FITC). The coloured bars assigned to specific clones correspond to their positions marked on cytogenetic maps in Figures 4 and 6. Chromosomes were counterstained with DAPI.
Figure 2.
Comparative BAC-FISH mapping of clones from the chromosome Bd3 to various species of Brachypodium.
Only one chromosome of a homeologous pair is shown in each cell. The colour of the text label in the first column indicates the fluorochrome used (red – tetramethylrhodamine, green – FITC). The coloured bars assigned to specific clones correspond to their positions marked on cytogenetic maps in Figures 5 and 7. Chromosomes were counterstained with DAPI.
Figure 3.
Cytogenetic maps of chromosomes bearing regions homeologous to chromosome Bd2 in various Brachypodium species.
Coloured bars mark the chromosomal positions of specific BAC clones assigned to the chromosome Bd2 in B. distachyon (A), B. sylvaticum (B), B. pinnatum 2n = 18 (C), B. pinnatum 2n = 28 (D), B. phoenicoides (E). Colour codes for the clones used in the study (F). The position of the clones on the diagram (A) reflects their localisation on the physical map [32].
Figure 4.
Positions of clones used in reference to the regions of the chromosome Bd2 homeologous with rice chromosomes.
Coloured bars mark the chromosomal positions of specific BAC clones assigned to the chromosome Bd2. A cytogenetic map of B. distachyon chromosome Bd2 linked with the Bd2 assembly schematics showing the regions that correspond to different rice chromosomes (A) (adapted from [9]), Colour codes for the clones used in the study (B), Colour codes for rice chromosomes homologous to the chromosome Bd2 (C). Black diamonds identify the positions of the fusion points in the Bd2. Dashed lines mark the chromosomal breakpoints found in B. sylvaticum, B. pinnatum 2n = 18 and 2n = 28, and in B. phoenicoides. A dotted line marks the breakpoint specific for B. sylvaticum. The position of the clones on the diagram (A) reflects their localisation on the physical map [32].
Figure 5.
Comparative BAC-FISH mapping of the clones from chromosomes Bd2 and Bd3 to B. sylvaticum (A–B), B. pinnatum 2n = 18 (C–D), B. pinnatum 2n = 28 (E–F) and B. phoenicoides (G–H).
BACs Bd2/1 – green and Bd2/10 – red (A), BACs Bd2/4 – red and Bd2/9 – green (C, E, G), BACs Bd3/1 – red and Bd3/6 – green (B, D, F, H). Chromosomes were counterstained with DAPI. Bar: 5 µm.
Figure 6.
Cytogenetic maps of the chromosomes bearing regions homeologous to the chromosome Bd3 in various Brachypodium species.
Coloured bars mark the chromosomal positions of specific BAC clones assigned to the chromosome Bd3 in B. distachyon (A), B. sylvaticum (B), B. phoenicoides (C), B. pinnatum 2n = 18 (D), B. pinnatum 2n = 28 (E). Colour codes for the clones used in the study (F). The position of the clones on the diagram (A) reflects their localisation on the physical map [32].
Figure 7.
Positions of the clones used in reference to the regions of the chromosome Bd2 homeologous with rice chromosomes.
Coloured bars mark the chromosomal positions of specific BAC clones assigned to the chromosome Bd3. A cytogenetic map of B. distachyon chromosome Bd3 linked with the Bd3 assembly schematics showing the regions that correspond to different rice chromosomes (A) (adapted from [9]), Colour codes for the clones used in the study (B), Colour codes for the rice chromosomes homologous to the chromosome Bd3 (C). Black diamonds identify the positions of the fusion points in the Bd3. Dashed lines mark the chromosomal breakpoints found in B. sylvaticum, B. pinnatum 2n = 18 and 2n = 28 and in B. phoenicoides. The position of the clones on the diagram (A) reflects their localisation on the physical map [32].