Table 1.
Mean values (± standard deviation) of the physicochemical parameters of goat milk produced by the Alpine and Saanen breeds in Northeastern Brazil.
Figure 1.
Electrophoretic analyses of goat milk proteins produced by the Alpine and Saanen breeds in Northeastern Brazil precipitated with ammonium sulfate, analyzed by gradient (7 to 20%) SDS-PAGE.
Image A: Alpine breed. (M) Molecular markers*, (A) Crude protein extract, (B) Fraction F0−30%, (C) Fraction F30−60%, (D) Fraction F60−90%, and (E) Fraction F90−100%. Image B: Saanen breed. (M) Molecular Markers*, (A) Crude protein extract (B) Fraction F0−30%, (C) Fraction F30−60%, (D) Fraction F60−90%, and (E) Fraction F90−100%. * BSA (66.0 kDa), egg albumin (45.0 kDa), carbonic anhydrase (30.0 kDa), trypsin inhibitor (23.3 kDa) and egg lysozyme (14.0 kDa).
Figure 2.
Gradient SDS-PAGE (7 to 20%) analyses of goat milk produced by the Alpine and Saanen breeds in Northeastern Brazil.
(M) Molecular markers*, (A) Alpine crude protein extract, (B) Saanen crude protein extract, (C) casein concentrate from Alpine milk, and (D) casein concentrate from Saanen milk. * BSA (66.0 kDa), egg albumin (45.0 kDa), carbonic anhydrase (30.0 kDa), trypsin inhibitor (23.3 kDa) and egg lysozyme (14 kDa).
Figure 3.
Chromatograms of casein concentrates of goat milk produced by the Alpine and Saanen breeds in Northeastern Brazil using RP-HPLC.
Image A: Casein concentrate from Alpine breed. Image B: Casein concentrate from Saanen breed. (1) κ-casein, (2) αs2-casein, and (3) β-casein.
Figure 4.
Comparison of two-dimensional electrophoresis of goat crude protein extract milk produced by the Alpine (AM) and Saanen (SM) breeds in Northeastern Brazil.
The vertical direction shows the molecular mass in kDa, and the horizontal direction shows the isoelectric focusing at pH 4–7. A: Molecular markers: BSA (66.0 kDa), egg albumin (45.0 kDa), carbonic anhydrase (30.0 kDa), trypsin inhibitor (23.3 kDa) and egg lysozyme (14.0 kDa). Gels were Coomassi stained.