Table 1.
Characteristics of participants for phagocytosis assays by type 2 diabetes status.
Figure 1.
Correlations between FcγR- and CR-mediated phagocytosis and host characteristics.
sRBCs were coated with C3b/iC3b or IgG and the percentage of monocytes with at least one phagocytosed sRBC was established by fluorescence microscopy among participants with and without DM2. The correlation between phagocytosis via CRs for C3b/iC3b-coated sRBCs and via FcγRs for IgG-coated sRBCs is shown with respect to HbA1c, or two other host characteristics that are also distinct in DM2 patients: age and BMI. Correlation coefficients (R) and corresponding p values are provided, and the linear regression displayed. Black circles, no DM2; gray circles, DM2.
Table 2.
Relationship between host characteristics and percent of MN phagocytosis of sheep red blood cells coated with antibodies or complement.
Figure 2.
Correlation between CR- and FcγR-mediated phagocytosis.
Phagocytosis of sRBCs coated with C3b/iC3b or IgG is described in the Methods and the linear relationship between both phagocytosis pathways is displayed. Correlation coefficients (R) and corresponding p value is provided. Black circles, no DM2; gray circles, DM2.
Figure 3.
Relationship between host factors and the median fluorescence intensity (MFI) of phagocytosis-promoting and -inhibiting receptors.
The mean of the MFI for monocyte receptors was established for all the CD14-positive monocytes or the classical, intermediate and non-classical monocytes as described in the Methods and shown in Table S2. Three host factors were associated with changes by univariable and multivariable analysis among all monocytes (gender, BMI and DM2 in columns), and their relationship with CD32, CD64 and CD32B is illustrated (rows): male gender with decreased CD32 but not CD64 or CD32B, higher BMI with increased CD64 but not CD32 or CD32B, and DM2 with increased CD32B expression. Correlation coefficients (R) and corresponding p values are provided, and the linear relationship displayed for BMI. Horizontal line shows mean by DM2 status.