Table 1.
Collection information for samples used in this study.
Table 2.
Universal primer, dye and group of microsatellite primers of Tetranychus species used in this study.
Table 3.
Universal primer and dye of microsatellite primers of F. occidentalis and N. lugens used in this study.
Figure 1.
Multiplex PCR results of T. truncatus BC02.
Three panels refer to singleplex, samples amplified by PA and PB separately then were pooled for capillary loading and samples amplified by PA+B respectively. P1: TUFG130N. V1: TUFG55. F1: TUFG177. N1: TUFG127. V2: TUFG57. N2: TUFG146. P2: TUFG147N. F2: TUFG87.
Figure 2.
Multiplex PCR results of F. occidentalis HB04.
Two panels refer to singleplex and sample amplified by ten loci in one multiplex PCR respectively. P1: WFT28. N1: WFT66. V1: WFT108. N2: WFT104. F1: WFT20. P2: WFT98. V2: WFT83. F2: WFT83. V3: WFT64. F3: WFT51.
Figure 3.
Microsatellite profiles of DNA XZ04 amplified by four loci using three methods generated on a 3130 capillary sequencer.
a) locus TUFG177; b) locus TUFG87; c) locus TUFG57; d) locus TUFG55. Each part of figure contains three screenshots, which here represent singleplex, samples amplified by PA and PB then were pooled for capillary loading and samples amplified by PA+B using the method described herein.
Figure 4.
Microsatellite profiles of two DNA samples amplified by loci TkMS010 (left peaks) and TkMS013 (right peaks) through three methods generated on 3130 capillary sequencer.
a) DNA sample PZ02; b) DNA sample PZ04. Each part of the figure contains three screenshots which referred to singleplex, samples amplified by PE and PF then pooled for capillary loading and samples amplified by PE+F using the method described herein.