Table 1.
Orthoreoviruses and accession numbers used in the present study.
Figure 1.
Characteristics of a novel orthoreovirus, Miyazaki-Bali/2007, CPE in Vero E6 cells induced by infection (A), electron microscopic morphology (B), and separation patterns of dsRNA separated by 10% SDS-PAGE electrophoresis (C) in comparison with those of ARV, MRV1 and MRV3.
L2 and L3 segments, and M2 and M3 are considered to have co-migrated, forming one bond (*).
Table 2.
Homology in nucleotide and amino acid sequences of Sigma C protein (S1-segment), major inner capsid protein (S2-segment), sigma NS protein (S3-segment), and major outer capsid protein (S4-segment) between the orthoviruses of Nelson Bay group as well as avian, baboon and mammalian orthoreoviruses.
Figure 2.
Phylogenetic trees showing the phylogenetic positions of the Miyazaki-Bali/2007 strain and other orthoreovirus strains based on deduced amino acid sequence of the sigma C protein (A), major inner capsid protein (B), sigma NS protein (C), and major outer capsid protein (D) listed in Table 1.
Bootstrap values for each node are shown by the robustness of the tree. Miyazaki-Bali/2007, the strain isolated in the patient of the present study, is indicated in red font.
Table 3.
Summary of the orthoreoviruses belong to the species of Nelson Bay orthoreovirus isolated from humans with RTI.